Conjugative gene transfer in marine cyanobacteria: Synechococcus sp., Synechocystis sp. and Pseudanabaena sp.

Koji Sode, Masahiro Tatara, Haruko Takeyama, J. Grant Burgess, Tadashi Matsunaga

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Versatility of gene transfer by transconjugation in marine cyanobacteria was demonstrated. In this study, seven different marine cyanobacteria were used as recipient cells. First, transconjugation was carried out using the mobilizable transposon (Tn 5) carrying plasmid pSUP1021. Transconjugants were observed in all marine cyanobacteria tested. Second, the broad-host-range vector pKT230 (IncQ) was tested for transconjugation. pKT230 has been successfully transferred in a marine cyanobacterium Synechococcus sp. NKBG15041C, and replicated as an autonomous replicon without alteration in the restriction enzyme pattern. A maximum transfer efficiency of 5.2 × 10-4 transconjugants/recipient cell was observed, when mating was performed on agar plates containing low salinity (0.015 m NaCl) medium. This is the first study to demonstrate gene transfer in marine cyanobacteria via transconjugation.

Original languageEnglish
Pages (from-to)369-373
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume37
Issue number3
DOIs
Publication statusPublished - 1992 Jun
Externally publishedYes

Fingerprint

Synechococcus
Gene transfer
Synechocystis
Cyanobacteria
Genes
Replicon
Host Specificity
Salinity
Agar
Plasmids
Enzymes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology
  • Bioengineering
  • Biotechnology

Cite this

Conjugative gene transfer in marine cyanobacteria : Synechococcus sp., Synechocystis sp. and Pseudanabaena sp. / Sode, Koji; Tatara, Masahiro; Takeyama, Haruko; Burgess, J. Grant; Matsunaga, Tadashi.

In: Applied Microbiology and Biotechnology, Vol. 37, No. 3, 06.1992, p. 369-373.

Research output: Contribution to journalArticle

Sode, Koji ; Tatara, Masahiro ; Takeyama, Haruko ; Burgess, J. Grant ; Matsunaga, Tadashi. / Conjugative gene transfer in marine cyanobacteria : Synechococcus sp., Synechocystis sp. and Pseudanabaena sp. In: Applied Microbiology and Biotechnology. 1992 ; Vol. 37, No. 3. pp. 369-373.
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