Connectable DNA-logic operation using droplets and rupture/reformation of bilayer lipid membranes

K. Inoue, R. Kawano, Hiroki Yasuga, M. Takinoue, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper experimentally demonstrated the connection of logic gates using DNA and membrane protein's channel reconstituted into bilayer lipid membranes (BLMs). In this system, droplets worked as the carrier of DNA to convey it as biological signal to next stage and reformation and rupture of BLM accelerated DNA transfer between droplets. Furthermore, DNA is detected based on the electrical and physical characters of DNA translocation via membrane protein's channel. In this study, we successfully demonstrated NOR operation by connecting OR and NOT gate. This system is readily applicable to develop high sensitive biosensor and computing system imitating biological information processing.

Original languageEnglish
Title of host publication2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages739-742
Number of pages4
ISBN (Electronic)9781479989553
DOIs
Publication statusPublished - 2015 Aug 5
Externally publishedYes
Event18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States
Duration: 2015 Jun 212015 Jun 25

Publication series

Name2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

Other

Other18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
CountryUnited States
CityAnchorage
Period15/6/2115/6/25

Fingerprint

Lipid bilayers
logic
lipids
DNA
deoxyribonucleic acid
membranes
proteins
Proteins
Membranes
Logic gates
Biological systems
bioinstrumentation
Biosensors
Membrane Lipids

Keywords

  • DNA computing
  • hybridization
  • Logic gate
  • Membrane protein
  • Planar lipid bilayer
  • Single molecule detection

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Inoue, K., Kawano, R., Yasuga, H., Takinoue, M., Osaki, T., Kamiya, K., ... Takeuchi, S. (2015). Connectable DNA-logic operation using droplets and rupture/reformation of bilayer lipid membranes. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 (pp. 739-742). [7181029] (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2015.7181029

Connectable DNA-logic operation using droplets and rupture/reformation of bilayer lipid membranes. / Inoue, K.; Kawano, R.; Yasuga, Hiroki; Takinoue, M.; Osaki, T.; Kamiya, K.; Miki, N.; Takeuchi, S.

2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. p. 739-742 7181029 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Inoue, K, Kawano, R, Yasuga, H, Takinoue, M, Osaki, T, Kamiya, K, Miki, N & Takeuchi, S 2015, Connectable DNA-logic operation using droplets and rupture/reformation of bilayer lipid membranes. in 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015., 7181029, 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Institute of Electrical and Electronics Engineers Inc., pp. 739-742, 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Anchorage, United States, 15/6/21. https://doi.org/10.1109/TRANSDUCERS.2015.7181029
Inoue K, Kawano R, Yasuga H, Takinoue M, Osaki T, Kamiya K et al. Connectable DNA-logic operation using droplets and rupture/reformation of bilayer lipid membranes. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc. 2015. p. 739-742. 7181029. (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). https://doi.org/10.1109/TRANSDUCERS.2015.7181029
Inoue, K. ; Kawano, R. ; Yasuga, Hiroki ; Takinoue, M. ; Osaki, T. ; Kamiya, K. ; Miki, N. ; Takeuchi, S. / Connectable DNA-logic operation using droplets and rupture/reformation of bilayer lipid membranes. 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 739-742 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).
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