Conservation of DNA bend sites with identical superhelical twists among the human, mouse, bovine, rabbit and chicken β-globin genes

Chanane Wanapirak, Yoshiaki Onishi, Yuko Wada-Kiyama, Takashi Ohyama, Ryoiti Kiyama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We reported previously that DNA bend sites appear in the human β-globin locus at an average distance of 680 bp. The relative locations of the sites were conserved among the five active β-like globin genes and one pseudogene. Here, we mapped the sites in the β-like globin genes from various species and examined their conservation. The locations of the bend sites in the bovine, rabbit and chicken β-globin genes mapped here showed marked conservation in their locations relative to the cap site and showed similar locations to the previously mapped sites in the human β- and mouse βmaj-globin genes. Further analysis of the first bend sites from the cap site (B-1 sites) indicated that they contained tracts of adenines and thymines longer than or equal to two bases. This sequence feature contributed mostly to the curvature profiles revealed by gel assays and/or by computer-based TRIF analysis. TRIF analysis indicated that most of the B-1 sites showed right-handed superhelical twists accompanied by left handed twists. This was confirmed by the effect of ethidium bromide on the superhelical twists in the assays.

Original languageEnglish
Pages (from-to)253-259
Number of pages7
JournalDNA Research
Volume7
Issue number4
DOIs
Publication statusPublished - 2000 Jan 1

Keywords

  • Bent DNA
  • Curvature
  • Evolution
  • Globin gene
  • Superhelicity

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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