We developed three techniques to make artificial neuronal networks constructed from rat hippocampal neurons. 1) a method of non-invasively collecting primary cultured neurons and their deposition, 2) a technique for microprocessing agarose for the purpose of assembling artificial neuronal networks, 3) a multi-electrode array system for measurement of the multi-point extracellular potential of neurons. The three techniques allow us to assemble and evaluate artificial neuronal networks constructed from particular cells. We can manipulate neuro-transmission pathways and investigate roles played by the innate period or stability information for each individual cell in the framework of physiological mechanism. It is thus possible to construct and demonstrated the actual neuronal networks simulated by the computed neural networks.