Construction of a genetic AND gate under a new standard for assembly of genetic parts

Shotaro Ayukawa, Akio Kobayashi, Yusaku Nakashima, Hidemasa Takagi, Shogo Hamada, Masahiko Uchiyama, Katsuyuki Yugi, Satoshi Murata, Yasubumi Sakakibara, Masami Hagiya, Masayuki Yamamura, Daisuke Kiga

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Appropriate regulation of respective gene expressions is a bottleneck for the realization of artificial biological systems inside living cells. The modification of several promoter sequences is required to achieve appropriate regulation of the systems. However, a time-consuming process is required for the insertion of an operator, a binding site of a protein for gene expression, to the gene regulatory region of a plasmid. Thus, a standardized method for integrating operator sequences to the regulatory region of a plasmid is required.Results: We developed a standardized method for integrating operator sequences to the regulatory region of a plasmid and constructed a synthetic promoter that functions as a genetic AND gate. By standardizing the regulatory region of a plasmid and the operator parts, we established a platform for modular assembly of the operator parts. Moreover, by assembling two different operator parts on the regulatory region, we constructed a regulatory device with an AND gate function.Conclusions: We implemented a new standard to assemble operator parts for construction of functional genetic logic gates. The logic gates at the molecular scale have important implications for reprogramming cellular behavior.

Original languageEnglish
Article numberS16
JournalBMC Genomics
Volume11
Issue numberSUPPL. 4
DOIs
Publication statusPublished - 2010 Dec 2

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

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    Ayukawa, S., Kobayashi, A., Nakashima, Y., Takagi, H., Hamada, S., Uchiyama, M., Yugi, K., Murata, S., Sakakibara, Y., Hagiya, M., Yamamura, M., & Kiga, D. (2010). Construction of a genetic AND gate under a new standard for assembly of genetic parts. BMC Genomics, 11(SUPPL. 4), [S16]. https://doi.org/10.1186/1471-2164-11-S4-S16