Construction of a nanosecond fluorometric system for applications to biological samples at cell or tissue levels

Kazuhiko Kinosita, Shigeki Mitaku, Akira Ikegami, Nobuo Ohbo, Tosiyasu L. Kunii

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A fluorometric system utilizing the single photon counting technique which records the decay of the intensity and the anisotropy of fluorescence following pulse excitation is described. Both macroscopic observations on cellular suspensions and measurements on individual cells under an optical microscope are possible. The system is so designed to permit precise evaluation of complex anisotropy decays, which is essential for studies of cells or tissues. Data are processed by a method which properly analyzes these complex decays. A preliminary study on erythrocyte membrane has confirmed the high resolution and precision of the system.

Original languageEnglish
Pages (from-to)1391-1392
Number of pages2
JournalJapanese Journal of Applied Physics
Volume15
Issue number12
DOIs
Publication statusPublished - 1976
Externally publishedYes

Fingerprint

Anisotropy
Tissue
decay
cells
anisotropy
Microscopes
Photons
Fluorescence
erythrocytes
optical microscopes
Membranes
counting
membranes
fluorescence
evaluation
high resolution
photons
pulses
excitation

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Construction of a nanosecond fluorometric system for applications to biological samples at cell or tissue levels. / Kinosita, Kazuhiko; Mitaku, Shigeki; Ikegami, Akira; Ohbo, Nobuo; Kunii, Tosiyasu L.

In: Japanese Journal of Applied Physics, Vol. 15, No. 12, 1976, p. 1391-1392.

Research output: Contribution to journalArticle

Kinosita, Kazuhiko ; Mitaku, Shigeki ; Ikegami, Akira ; Ohbo, Nobuo ; Kunii, Tosiyasu L. / Construction of a nanosecond fluorometric system for applications to biological samples at cell or tissue levels. In: Japanese Journal of Applied Physics. 1976 ; Vol. 15, No. 12. pp. 1391-1392.
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