TY - JOUR
T1 - Construction of a nanosecond fluorometric system for applications to biological samples at cell or tissue levels
AU - Kinosita, Kazuhiko
AU - Mitaku, Shigeki
AU - Ikegami, Akira
AU - Ohbo, Nobuo
AU - Kunii, Tosiyasu L.
PY - 1976
Y1 - 1976
N2 - A fluorometric system utilizing the single photon counting technique which records the decay of the intensity and the anisotropy of fluorescence following pulse excitation is described. Both macroscopic observations on cellular suspensions and measurements on individual cells under an optical microscope are possible. The system is so designed to permit precise evaluation of complex anisotropy decays, which is essential for studies of cells or tissues. Data are processed by a method which properly analyzes these complex decays. A preliminary study on erythrocyte membrane has confirmed the high resolution and precision of the system.
AB - A fluorometric system utilizing the single photon counting technique which records the decay of the intensity and the anisotropy of fluorescence following pulse excitation is described. Both macroscopic observations on cellular suspensions and measurements on individual cells under an optical microscope are possible. The system is so designed to permit precise evaluation of complex anisotropy decays, which is essential for studies of cells or tissues. Data are processed by a method which properly analyzes these complex decays. A preliminary study on erythrocyte membrane has confirmed the high resolution and precision of the system.
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U2 - 10.1143/JJAP.15.2433
DO - 10.1143/JJAP.15.2433
M3 - Article
AN - SCOPUS:84956089198
SN - 0021-4922
VL - 15
SP - 1391
EP - 1392
JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
IS - 12
ER -