Construction of a potato fraction library for the investigation of functional secondary metabolites

Toshihiko Nogawa, Yushi Futamura, Akiko Okano, Mari Suto, Junya Nakamura, Katsuyuki Ishihara, Hiroyuki Osada*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


A potato fraction library was constructed to investigate functional secondary metabolites from 8 cultivars: Kitahime, Pilka, Sakurafubuki, Atlantic, Toyoshiro, Snowden, Kitamurasaki, and Northern Ruby, which were divided into flower, leaf, stem, roots, tuber peel, and tuber. Each fraction was a semi-purified extract and about 800 fractions were prepared for the library. They were analyzed by DAD-LC/MS to obtain structural information and were evaluated for various biological activities. LC/MS data showed that each part had a specific characteristic for their constituents supported by principal component analysis (PCA). Approximately 40% of fractions showed significant biological activities at 30 μg/mL, especially the flower fractions showed strong cytotoxicity. PCAs based on the activity and LC/MS data suggested that the strong cytotoxicity of flowers was derived from a complex mixture of potato glycoalkaloids. In addition, tuber peel fractions showed strong antimalarial activity, which had not been reported before. Also, some fractions showed significant antibacterial activities.

Original languageEnglish
Pages (from-to)65-75
Number of pages11
JournalBioscience, Biotechnology and Biochemistry
Issue number1
Publication statusPublished - 2019
Externally publishedYes


  • Fraction library
  • LC/MS
  • Potato
  • Secondary metabolite

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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