Abstract
Miniaturized immunosensors, that combine the analytical power of microfluidic devices with the high specificity of biomolecules, have been intensively studied for clinical analyses, environmental immunoassays and biochemical studies. Protein A has the ability to bind Fc-binding site of immunoglobulin G (IgG), and can be used as the detection probe in immunoassays. Ferrocenecarboaldehyde (Fc-CHO) was labeled to protein A as an electrochemical probe for on chip type amperometric flow immunoassay. When labeling Fc-CHO onto protein A via formation of Schiff-base and its reduction, mean number of nine Fc-CHO labeled to individual protein A molecules. Protein A labeled with Fc-CHO exhibited reversible redox properties of ferrocene in cyclic voltammograms and its peak currents increased with number of labeled ferrocenes. The current response of protein A labeled with Fc-CHO well correlated with its concentration when applying 390 V versus Ag/AgCl. Protein A labeled with Fc-CHO showed sufficient electrochemical properties that are useful in a miniaturized electrochemical immunoassays.
| Original language | English |
|---|---|
| Pages (from-to) | 952-955 |
| Number of pages | 4 |
| Journal | Electrochimica Acta |
| Volume | 51 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2005 Nov 10 |
| Externally published | Yes |
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Keywords
- Electrochemical probe
- Ferrocene derivatives
- Immunoassay
- Protein A
ASJC Scopus subject areas
- Chemical Engineering(all)
- Electrochemistry
Cite this
Construction of an electrochemical probe for on chip type flow immunoassay. / Okochi, Mina; Ohta, Hiroko; Taguchi, Tomoyuki; Ohta, Hiroyuki; Matsunaga, Tadashi.
In: Electrochimica Acta, Vol. 51, No. 5, 10.11.2005, p. 952-955.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Construction of an electrochemical probe for on chip type flow immunoassay
AU - Okochi, Mina
AU - Ohta, Hiroko
AU - Taguchi, Tomoyuki
AU - Ohta, Hiroyuki
AU - Matsunaga, Tadashi
PY - 2005/11/10
Y1 - 2005/11/10
N2 - Miniaturized immunosensors, that combine the analytical power of microfluidic devices with the high specificity of biomolecules, have been intensively studied for clinical analyses, environmental immunoassays and biochemical studies. Protein A has the ability to bind Fc-binding site of immunoglobulin G (IgG), and can be used as the detection probe in immunoassays. Ferrocenecarboaldehyde (Fc-CHO) was labeled to protein A as an electrochemical probe for on chip type amperometric flow immunoassay. When labeling Fc-CHO onto protein A via formation of Schiff-base and its reduction, mean number of nine Fc-CHO labeled to individual protein A molecules. Protein A labeled with Fc-CHO exhibited reversible redox properties of ferrocene in cyclic voltammograms and its peak currents increased with number of labeled ferrocenes. The current response of protein A labeled with Fc-CHO well correlated with its concentration when applying 390 V versus Ag/AgCl. Protein A labeled with Fc-CHO showed sufficient electrochemical properties that are useful in a miniaturized electrochemical immunoassays.
AB - Miniaturized immunosensors, that combine the analytical power of microfluidic devices with the high specificity of biomolecules, have been intensively studied for clinical analyses, environmental immunoassays and biochemical studies. Protein A has the ability to bind Fc-binding site of immunoglobulin G (IgG), and can be used as the detection probe in immunoassays. Ferrocenecarboaldehyde (Fc-CHO) was labeled to protein A as an electrochemical probe for on chip type amperometric flow immunoassay. When labeling Fc-CHO onto protein A via formation of Schiff-base and its reduction, mean number of nine Fc-CHO labeled to individual protein A molecules. Protein A labeled with Fc-CHO exhibited reversible redox properties of ferrocene in cyclic voltammograms and its peak currents increased with number of labeled ferrocenes. The current response of protein A labeled with Fc-CHO well correlated with its concentration when applying 390 V versus Ag/AgCl. Protein A labeled with Fc-CHO showed sufficient electrochemical properties that are useful in a miniaturized electrochemical immunoassays.
KW - Electrochemical probe
KW - Ferrocene derivatives
KW - Immunoassay
KW - Protein A
UR - http://www.scopus.com/inward/record.url?scp=27644530593&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27644530593&partnerID=8YFLogxK
U2 - 10.1016/j.electacta.2005.04.075
DO - 10.1016/j.electacta.2005.04.075
M3 - Article
AN - SCOPUS:27644530593
VL - 51
SP - 952
EP - 955
JO - Electrochimica Acta
JF - Electrochimica Acta
SN - 0013-4686
IS - 5
ER -