Abstract
Miniaturized immunosensors, that combine the analytical power of microfluidic devices with the high specificity of biomolecules, have been intensively studied for clinical analyses, environmental immunoassays and biochemical studies. Protein A has the ability to bind Fc-binding site of immunoglobulin G (IgG), and can be used as the detection probe in immunoassays. Ferrocenecarboaldehyde (Fc-CHO) was labeled to protein A as an electrochemical probe for on chip type amperometric flow immunoassay. When labeling Fc-CHO onto protein A via formation of Schiff-base and its reduction, mean number of nine Fc-CHO labeled to individual protein A molecules. Protein A labeled with Fc-CHO exhibited reversible redox properties of ferrocene in cyclic voltammograms and its peak currents increased with number of labeled ferrocenes. The current response of protein A labeled with Fc-CHO well correlated with its concentration when applying 390 V versus Ag/AgCl. Protein A labeled with Fc-CHO showed sufficient electrochemical properties that are useful in a miniaturized electrochemical immunoassays.
Original language | English |
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Pages (from-to) | 952-955 |
Number of pages | 4 |
Journal | Electrochimica Acta |
Volume | 51 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2005 Nov 10 |
Externally published | Yes |
Keywords
- Electrochemical probe
- Ferrocene derivatives
- Immunoassay
- Protein A
ASJC Scopus subject areas
- Chemical Engineering(all)
- Electrochemistry