Construction of artificial methemoglobin reduction systems in Hb vesicles

Shinji Takeoka, Tekeru Ohgushi, Hiromi Sakai, Takehiro Kose, Hiroyuki Nishide, Eishun Tsuchida

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Abstract

The hemoglobin vesicle (HbV) is a red cell substitute encapsulating purified concentrated Hb in a phospholipid vesicle. In order to suppress metHb formation for the long term maintenance of oxygen transporting capability in vivo, thiols (cysteine, Cys; homocysteine, Hcy) were studied as reductants of metHb. Hcy showed a suppressive effect on metHb formation, while Cys adversely accelerates metHb formation at the rate of twice the Hb solution without any reductants. The suppression of Cys-induced metHb formation by the addition of superoxide dismutase (SOD) and catalase indicated that Cys was easily oxidized by oxygen and simultaneously generated a large amount of active oxygens. The rate of metHb formation was influenced by PO2 and pH. Furthermore, the reducing systems (methylene blue (MB), NADH or ascorbic acid) were added to the outer aqueous phase of HbV, and the artificial reduction systems constructed through the bilayer membrane were evaluated.

Original languageEnglish
Pages (from-to)31-41
Number of pages11
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume25
Issue number1-2
DOIs
Publication statusPublished - 1997 Jan 1

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ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering

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