Construction of manganese porphyrin modified hemoglobin complex and its antioxidant activities

Hidenori Murata, Yuji Ito, Tomoyuki Shimpo, Kenichi Oyaizu, Makoto Yuasa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Manganese 5,10,15,20-tetrakis(N-methyl-2-yl) porphyrin (MnT2MePyP) was introduced to hemoglobin to use it as a drug carrier. Reconstituted hemoglobin was reacted with pyridoxal-5-phosphate and borohydride to yield pyridoxalated reconstituted hemoglobin (PLPrHb). The reaction of PLPrHb with activated polyethyleneglycol gave pegylated reconstituted hemoglobin (PEGrHb). PEGrHb was polymerized using glutaraldehyde to yield cross-linked pegylated reconstituted hemoglobin (Cross-link PEGrHb). The number average moleculer weight of Cross-link PEGrHb was estimated to be 1.0 _Xt 105 by GPC. The modification of hemoglobin was checked by using SDS-PAGE. Stopped flow analysis showed a high SOD activity of Cross-link PEGrHb. A pharmacokinetic study of 125I-labelled Cross-link PEGrHb that was infused into mice revealed a long-term blood circulation. The novel Manganese porphyrin modified hemoglobin complex was found to remain long-term in the blood circulation without being removed by excretion. These results showed that this complex can be used as a high performance antioxidant.

Original languageEnglish
Pages (from-to)277-282
Number of pages6
JournalKOBUNSHI RONBUNSHU
Volume65
Issue number4
DOIs
Publication statusPublished - 2008 Apr 1
Externally publishedYes

Keywords

  • Antioxidant
  • Cross-link
  • Hemoglobin
  • Manganese porphyrin

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Materials Science (miscellaneous)
  • Environmental Science(all)
  • Polymers and Plastics

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