Construction of photoenergetic mitochondria in cultured mammalian cells.

Kiyotaka Y. Hara, Takeyoshi Wada, Kuniki Kino, Toru Asahi, Naoya Sawamura

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The proton motive force (PMF) is bio-energetically important for various cellular reactions to occur. We developed PMF-photogenerating mitochondria in cultured mammalian cells. An archaebacterial rhodopsin, delta-rhodopsin, which is a light-driven proton pump derived from Haloterrigena turkmenica, was expressed in the mitochondria of CHO-K1 cells. The constructed stable CHO-K1 cell lines showed suppression of cell death induced by rotenone, a pesticide that inhibits mitochondrial complex I activity involved in PMF generation through the electron transport chain. Delta-rhodopsin was also introduced into the mitochondria of human neuroblastoma SH-SY5Y cells. The constructed stable SH-SY5Y cell lines showed suppression of dopaminergic neuronal cell death induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), an inducer of Parkinson's disease models, which acts through inhibition of complex I activity. These results suggest that the light-activated proton pump functioned as a PMF generator in the mitochondria of mammalian cells, and suppressed cell death induced by inhibition of respiratory PMF generation.

Original languageEnglish
Pages (from-to)1635
Number of pages1
JournalScientific Reports
Volume3
Publication statusPublished - 2013

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Proton-Motive Force
Cultured Cells
Mitochondria
Rhodopsin
Proton Pumps
Cell Death
CHO Cells
Light
Rotenone
Cell Line
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Electron Transport
Neuroblastoma
Pesticides
Parkinson Disease

ASJC Scopus subject areas

  • General

Cite this

Construction of photoenergetic mitochondria in cultured mammalian cells. / Hara, Kiyotaka Y.; Wada, Takeyoshi; Kino, Kuniki; Asahi, Toru; Sawamura, Naoya.

In: Scientific Reports, Vol. 3, 2013, p. 1635.

Research output: Contribution to journalArticle

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