Continuous concentration and separation of microparticles using dielectrophoretic force in a V-shaped electrode array

Masahito Hayashi, Tomoyuki Kaneko, Kenji Yasuda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have proposed and developed the novel principle of a V-shaped electrode array in a microfluidic pathway for continuous concentration and separation of particles by dielectrophoretic (DEP) force. The advantage of V-shape microelectrode arrays with a microfluidic flow for cell separation is that whole particles are concentrated into the center of a micropathway independent of the difference in their dielectric constants in the X-Y plane, while the particles are split between the top or bottom of the micropathway in the Z-axis direction depending on the differences in their dielectric constants and the applied AC frequency. After the application of a sinusoidal AC voltage of 1MHz and 20V pp, both polystyrene spheres and Bacillus subtilis spores were concentrated at the tip of the V-shaped electrode at the center of microfluidic flow in the X-Y plane independent of their dielectric constant differences. They were also split into two directions in the Z-axis, i.e., polystyrene spheres rose to the top, and spores went down to the bottom depending on their dielectric constant differences and were successfully separated in two layered downstreams. The results indicate the potential of V-shaped electrode arrays for simple continuous purification of mixed particles depending on their dielectric constants.

Original languageEnglish
Article number06GL03
JournalJapanese Journal of Applied Physics
Volume50
Issue number6 PART 2
DOIs
Publication statusPublished - 2011 Jun
Externally publishedYes

Fingerprint

microparticles
Permittivity
permittivity
Electrodes
electrodes
Microfluidics
spores
Polystyrenes
polystyrene
alternating current
Bacillus
Microelectrodes
Bacilli
purification
Purification
Electric potential
electric potential
cells

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Continuous concentration and separation of microparticles using dielectrophoretic force in a V-shaped electrode array. / Hayashi, Masahito; Kaneko, Tomoyuki; Yasuda, Kenji.

In: Japanese Journal of Applied Physics, Vol. 50, No. 6 PART 2, 06GL03, 06.2011.

Research output: Contribution to journalArticle

@article{189bcddc2f4243d8ab5d4241d6d7bcd9,
title = "Continuous concentration and separation of microparticles using dielectrophoretic force in a V-shaped electrode array",
abstract = "We have proposed and developed the novel principle of a V-shaped electrode array in a microfluidic pathway for continuous concentration and separation of particles by dielectrophoretic (DEP) force. The advantage of V-shape microelectrode arrays with a microfluidic flow for cell separation is that whole particles are concentrated into the center of a micropathway independent of the difference in their dielectric constants in the X-Y plane, while the particles are split between the top or bottom of the micropathway in the Z-axis direction depending on the differences in their dielectric constants and the applied AC frequency. After the application of a sinusoidal AC voltage of 1MHz and 20V pp, both polystyrene spheres and Bacillus subtilis spores were concentrated at the tip of the V-shaped electrode at the center of microfluidic flow in the X-Y plane independent of their dielectric constant differences. They were also split into two directions in the Z-axis, i.e., polystyrene spheres rose to the top, and spores went down to the bottom depending on their dielectric constant differences and were successfully separated in two layered downstreams. The results indicate the potential of V-shaped electrode arrays for simple continuous purification of mixed particles depending on their dielectric constants.",
author = "Masahito Hayashi and Tomoyuki Kaneko and Kenji Yasuda",
year = "2011",
month = "6",
doi = "10.1143/JJAP.50.06GL03",
language = "English",
volume = "50",
journal = "Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes",
issn = "0021-4922",
publisher = "Japan Society of Applied Physics",
number = "6 PART 2",

}

TY - JOUR

T1 - Continuous concentration and separation of microparticles using dielectrophoretic force in a V-shaped electrode array

AU - Hayashi, Masahito

AU - Kaneko, Tomoyuki

AU - Yasuda, Kenji

PY - 2011/6

Y1 - 2011/6

N2 - We have proposed and developed the novel principle of a V-shaped electrode array in a microfluidic pathway for continuous concentration and separation of particles by dielectrophoretic (DEP) force. The advantage of V-shape microelectrode arrays with a microfluidic flow for cell separation is that whole particles are concentrated into the center of a micropathway independent of the difference in their dielectric constants in the X-Y plane, while the particles are split between the top or bottom of the micropathway in the Z-axis direction depending on the differences in their dielectric constants and the applied AC frequency. After the application of a sinusoidal AC voltage of 1MHz and 20V pp, both polystyrene spheres and Bacillus subtilis spores were concentrated at the tip of the V-shaped electrode at the center of microfluidic flow in the X-Y plane independent of their dielectric constant differences. They were also split into two directions in the Z-axis, i.e., polystyrene spheres rose to the top, and spores went down to the bottom depending on their dielectric constant differences and were successfully separated in two layered downstreams. The results indicate the potential of V-shaped electrode arrays for simple continuous purification of mixed particles depending on their dielectric constants.

AB - We have proposed and developed the novel principle of a V-shaped electrode array in a microfluidic pathway for continuous concentration and separation of particles by dielectrophoretic (DEP) force. The advantage of V-shape microelectrode arrays with a microfluidic flow for cell separation is that whole particles are concentrated into the center of a micropathway independent of the difference in their dielectric constants in the X-Y plane, while the particles are split between the top or bottom of the micropathway in the Z-axis direction depending on the differences in their dielectric constants and the applied AC frequency. After the application of a sinusoidal AC voltage of 1MHz and 20V pp, both polystyrene spheres and Bacillus subtilis spores were concentrated at the tip of the V-shaped electrode at the center of microfluidic flow in the X-Y plane independent of their dielectric constant differences. They were also split into two directions in the Z-axis, i.e., polystyrene spheres rose to the top, and spores went down to the bottom depending on their dielectric constant differences and were successfully separated in two layered downstreams. The results indicate the potential of V-shaped electrode arrays for simple continuous purification of mixed particles depending on their dielectric constants.

UR - http://www.scopus.com/inward/record.url?scp=79959473103&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959473103&partnerID=8YFLogxK

U2 - 10.1143/JJAP.50.06GL03

DO - 10.1143/JJAP.50.06GL03

M3 - Article

VL - 50

JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

SN - 0021-4922

IS - 6 PART 2

M1 - 06GL03

ER -