Continuum of overlapping clones spanning the entire human chromosome 21q

Ilya Chumakov, Philippe Rigault, Sophie Guillou, Pierre Ougen, Alain Billaut, Ghislaine Guasconi, Patricia Gervy, Isabelle LeGall, Pascal Soularue, Laurent Grinas, Lydie Bougueleret, Christine Bellanné-Chantelot, Bruno Lacroix, Emmanuel Barillot, Philippe Gesnouin, Stuart Pook, Guy Vaysseix, Gerard Frelat, Annette Schmitz, Jean Luc SambucyAssumpcio Bosch, Xavier Estivill, Jean Weissenbach, Alain Vignal, Harold Riethman, David Cox, David Patterson, Kathleen Gardiner, Masahira Hattori, Yoshiyuki Sakaki, Hitoshi Ichikawa, Misao Ohki, Denis Le Paslier, Roland Heilig, Stylianos Antonarakis, Daniel Cohen

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Abstract

A continuous array of overlapping clones covering the entire human chromosome 21q was constructed from human yeast artificial chromosome libraries using sequence-tagged sites as landmarks specifically detected by polymerase chain reaction. The yeast artificial chromosome contiguous unit starts with pericentromeric and ends with subtelomeric loci of 21q. The resulting order of sequence-tagged sites is consistent with other physical and genetic mapping data. This set of overlapping clones will promote our knowledge of the structure of this chromosome and the function of its genes.

Original languageEnglish
Pages (from-to)380-387
Number of pages8
JournalNature
Volume359
Issue number6394
Publication statusPublished - 1992 Oct 1
Externally publishedYes

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Cite this

Chumakov, I., Rigault, P., Guillou, S., Ougen, P., Billaut, A., Guasconi, G., Gervy, P., LeGall, I., Soularue, P., Grinas, L., Bougueleret, L., Bellanné-Chantelot, C., Lacroix, B., Barillot, E., Gesnouin, P., Pook, S., Vaysseix, G., Frelat, G., Schmitz, A., ... Cohen, D. (1992). Continuum of overlapping clones spanning the entire human chromosome 21q. Nature, 359(6394), 380-387.