Contrary effects of octopamine receptor ligands on behavioral and neuronal changes in locomotion of lymnaea

Yurie Miyamae, Mami Komuro, Aya Murata, Kanako Aono, Kaori Nishikata, Akira Kanazawa, Yutaka Fujito, Takumi Komatsu, Daisuke Ito, Takashi Abe, Masafumi Nagayama, Tsutomu Uchida, Kazutoshi Gohara, Jun Murakami, Ryo Kawai, Dai Hatakeyama, Ken Lukowiak, Etsuro Ito

Research output: Contribution to journalArticle

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Abstract

The pond snail Lymnaea stagnalis moves along the sides and bottom of an aquarium, but it can also glide upside down on its back below the water's surface. We have termed these two forms of locomotion "standard locomotion" and "upside-down gliding," respectively. Previous studies showed that standard locomotion is produced by both cilia activity on the foot and peristaltic contraction of the foot muscles, whereas upside-down gliding is mainly caused by cilia activity. The pedal A neurons are thought to receive excitatory octopaminergic input, which ultimately results in increased cilia beating. However, the relationship between locomotory speed and the responses of these neurons to octopamine is not known. We thus examined the effects of both an agonist and an antagonist of octopamine receptors on locomotory speed and the firing rate of the pedal A neurons. We also examined, at the electron and light-microscopic levels, whether structural changes occur in cilia following the application of either an agonist or an antagonist of octopamine receptors to the central nervous system (CNS). We found that the application of an octopamine antagonist to the CNS increased the speed of both forms of locomotion, whereas application of octopamine increased only the ?ring rate of the pedal A neurons. Microscopic examination of the cilia proved that there were no changes in their morphology after application of octopamine ligands. These data suggest that there is an unidenti?ed octopaminergic neuronal network in the CNS whose acti-vation reduces cilia movement and thus locomotory speed.

Original languageEnglish
Pages (from-to)6-14
Number of pages9
JournalBiological Bulletin
Volume218
Issue number1
Publication statusPublished - 2010 Feb 1
Externally publishedYes

Fingerprint

Lymnaea
octopamine
Cilia
cilia
Locomotion
Octopamine
locomotion
Foot
Ligands
receptors
neurons
central nervous system
Neurons
antagonists
Central Nervous System
agonists
Lymnaea stagnalis
Snails
back (body region)
Muscle Contraction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Medicine(all)

Cite this

Miyamae, Y., Komuro, M., Murata, A., Aono, K., Nishikata, K., Kanazawa, A., ... Ito, E. (2010). Contrary effects of octopamine receptor ligands on behavioral and neuronal changes in locomotion of lymnaea. Biological Bulletin, 218(1), 6-14.

Contrary effects of octopamine receptor ligands on behavioral and neuronal changes in locomotion of lymnaea. / Miyamae, Yurie; Komuro, Mami; Murata, Aya; Aono, Kanako; Nishikata, Kaori; Kanazawa, Akira; Fujito, Yutaka; Komatsu, Takumi; Ito, Daisuke; Abe, Takashi; Nagayama, Masafumi; Uchida, Tsutomu; Gohara, Kazutoshi; Murakami, Jun; Kawai, Ryo; Hatakeyama, Dai; Lukowiak, Ken; Ito, Etsuro.

In: Biological Bulletin, Vol. 218, No. 1, 01.02.2010, p. 6-14.

Research output: Contribution to journalArticle

Miyamae, Y, Komuro, M, Murata, A, Aono, K, Nishikata, K, Kanazawa, A, Fujito, Y, Komatsu, T, Ito, D, Abe, T, Nagayama, M, Uchida, T, Gohara, K, Murakami, J, Kawai, R, Hatakeyama, D, Lukowiak, K & Ito, E 2010, 'Contrary effects of octopamine receptor ligands on behavioral and neuronal changes in locomotion of lymnaea', Biological Bulletin, vol. 218, no. 1, pp. 6-14.
Miyamae Y, Komuro M, Murata A, Aono K, Nishikata K, Kanazawa A et al. Contrary effects of octopamine receptor ligands on behavioral and neuronal changes in locomotion of lymnaea. Biological Bulletin. 2010 Feb 1;218(1):6-14.
Miyamae, Yurie ; Komuro, Mami ; Murata, Aya ; Aono, Kanako ; Nishikata, Kaori ; Kanazawa, Akira ; Fujito, Yutaka ; Komatsu, Takumi ; Ito, Daisuke ; Abe, Takashi ; Nagayama, Masafumi ; Uchida, Tsutomu ; Gohara, Kazutoshi ; Murakami, Jun ; Kawai, Ryo ; Hatakeyama, Dai ; Lukowiak, Ken ; Ito, Etsuro. / Contrary effects of octopamine receptor ligands on behavioral and neuronal changes in locomotion of lymnaea. In: Biological Bulletin. 2010 ; Vol. 218, No. 1. pp. 6-14.
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