Contribution of cyanide-insensitive respiratory pathway, catalyzed by the alternative oxidase, to citric acid production in Aspergillus niger

Kotaro Kirimura, Masashi Yoda, Hideki Shimizu, Shuji Sugano, Makoto Mizuno, Kuniki Kino, Shoji Usami

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    In Aspergillus niger, a cyanide (CN)- and antimycin A-insensitive and salicylhydroxamic acid (SHAM)-sensitive respiratory pathway exists besides the cytochrome pathway and is catalyzed by the alternative oxidase (AOX). In this study, A. niger WU-2223L, a citric acid-producing strain, was cultivated in a medium containing 120 g/l of glucose, which is the concentration usually needed for citric acid production, and the effects of 2% (v/v) methanol, an inducer of citric acid, 2 μM antimycin A, and 1 mM SHAM on AOX activities and citric acid production were investigated. The AOX activity, measured as duroquinol oxidase, was localized in the purified mitochondria regardless of the presence of any additives. When WU-2223L was cultivated with antimycin A or methanol, both citric acid production and citric acid productivity, shown as the ratio of production per mycelial dry weight, increased with the increase of both the activity of AOX and the rate of CN-insensitive and SHAM-sensitive respiration. On the other hand, when WU-2223L was cultivated with SHAM, an inhibitor of AOX, the CN-insensitive and SHAM-sensitive respiration was not detected and the citric acid production and the productivity drastically decreased, although mycelial growth was not affected. These results clearly indicated that the CN-insensitive and SHAM-sensitive respiration catalyzed by AOX, localized in the mitochondria, contributed to citric acid production by A. niger.

    Original languageEnglish
    Pages (from-to)2034-2039
    Number of pages6
    JournalBioscience, Biotechnology and Biochemistry
    Volume64
    Issue number10
    Publication statusPublished - 2000 Oct

    Fingerprint

    Aspergillus niger
    Aspergillus
    Citric acid
    Cyanides
    cyanides
    Citric Acid
    citric acid
    Antimycin A
    antimycin A
    Acids
    acids
    Respiration
    Mitochondria
    Methanol
    methanol
    mitochondria
    Productivity
    alternative oxidase
    Oxidoreductases
    Cytochromes

    Keywords

    • Alternative oxidase
    • Aspergillus niger
    • Citric acid
    • Cyanide-insensitive respiration
    • Mitochondria

    ASJC Scopus subject areas

    • Food Science
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry
    • Biotechnology
    • Chemistry (miscellaneous)
    • Applied Microbiology and Biotechnology
    • Bioengineering

    Cite this

    Contribution of cyanide-insensitive respiratory pathway, catalyzed by the alternative oxidase, to citric acid production in Aspergillus niger. / Kirimura, Kotaro; Yoda, Masashi; Shimizu, Hideki; Sugano, Shuji; Mizuno, Makoto; Kino, Kuniki; Usami, Shoji.

    In: Bioscience, Biotechnology and Biochemistry, Vol. 64, No. 10, 10.2000, p. 2034-2039.

    Research output: Contribution to journalArticle

    @article{6e7f46a1983e4c6383d55ea829507114,
    title = "Contribution of cyanide-insensitive respiratory pathway, catalyzed by the alternative oxidase, to citric acid production in Aspergillus niger",
    abstract = "In Aspergillus niger, a cyanide (CN)- and antimycin A-insensitive and salicylhydroxamic acid (SHAM)-sensitive respiratory pathway exists besides the cytochrome pathway and is catalyzed by the alternative oxidase (AOX). In this study, A. niger WU-2223L, a citric acid-producing strain, was cultivated in a medium containing 120 g/l of glucose, which is the concentration usually needed for citric acid production, and the effects of 2{\%} (v/v) methanol, an inducer of citric acid, 2 μM antimycin A, and 1 mM SHAM on AOX activities and citric acid production were investigated. The AOX activity, measured as duroquinol oxidase, was localized in the purified mitochondria regardless of the presence of any additives. When WU-2223L was cultivated with antimycin A or methanol, both citric acid production and citric acid productivity, shown as the ratio of production per mycelial dry weight, increased with the increase of both the activity of AOX and the rate of CN-insensitive and SHAM-sensitive respiration. On the other hand, when WU-2223L was cultivated with SHAM, an inhibitor of AOX, the CN-insensitive and SHAM-sensitive respiration was not detected and the citric acid production and the productivity drastically decreased, although mycelial growth was not affected. These results clearly indicated that the CN-insensitive and SHAM-sensitive respiration catalyzed by AOX, localized in the mitochondria, contributed to citric acid production by A. niger.",
    keywords = "Alternative oxidase, Aspergillus niger, Citric acid, Cyanide-insensitive respiration, Mitochondria",
    author = "Kotaro Kirimura and Masashi Yoda and Hideki Shimizu and Shuji Sugano and Makoto Mizuno and Kuniki Kino and Shoji Usami",
    year = "2000",
    month = "10",
    language = "English",
    volume = "64",
    pages = "2034--2039",
    journal = "Bioscience, Biotechnology and Biochemistry",
    issn = "0916-8451",
    publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
    number = "10",

    }

    TY - JOUR

    T1 - Contribution of cyanide-insensitive respiratory pathway, catalyzed by the alternative oxidase, to citric acid production in Aspergillus niger

    AU - Kirimura, Kotaro

    AU - Yoda, Masashi

    AU - Shimizu, Hideki

    AU - Sugano, Shuji

    AU - Mizuno, Makoto

    AU - Kino, Kuniki

    AU - Usami, Shoji

    PY - 2000/10

    Y1 - 2000/10

    N2 - In Aspergillus niger, a cyanide (CN)- and antimycin A-insensitive and salicylhydroxamic acid (SHAM)-sensitive respiratory pathway exists besides the cytochrome pathway and is catalyzed by the alternative oxidase (AOX). In this study, A. niger WU-2223L, a citric acid-producing strain, was cultivated in a medium containing 120 g/l of glucose, which is the concentration usually needed for citric acid production, and the effects of 2% (v/v) methanol, an inducer of citric acid, 2 μM antimycin A, and 1 mM SHAM on AOX activities and citric acid production were investigated. The AOX activity, measured as duroquinol oxidase, was localized in the purified mitochondria regardless of the presence of any additives. When WU-2223L was cultivated with antimycin A or methanol, both citric acid production and citric acid productivity, shown as the ratio of production per mycelial dry weight, increased with the increase of both the activity of AOX and the rate of CN-insensitive and SHAM-sensitive respiration. On the other hand, when WU-2223L was cultivated with SHAM, an inhibitor of AOX, the CN-insensitive and SHAM-sensitive respiration was not detected and the citric acid production and the productivity drastically decreased, although mycelial growth was not affected. These results clearly indicated that the CN-insensitive and SHAM-sensitive respiration catalyzed by AOX, localized in the mitochondria, contributed to citric acid production by A. niger.

    AB - In Aspergillus niger, a cyanide (CN)- and antimycin A-insensitive and salicylhydroxamic acid (SHAM)-sensitive respiratory pathway exists besides the cytochrome pathway and is catalyzed by the alternative oxidase (AOX). In this study, A. niger WU-2223L, a citric acid-producing strain, was cultivated in a medium containing 120 g/l of glucose, which is the concentration usually needed for citric acid production, and the effects of 2% (v/v) methanol, an inducer of citric acid, 2 μM antimycin A, and 1 mM SHAM on AOX activities and citric acid production were investigated. The AOX activity, measured as duroquinol oxidase, was localized in the purified mitochondria regardless of the presence of any additives. When WU-2223L was cultivated with antimycin A or methanol, both citric acid production and citric acid productivity, shown as the ratio of production per mycelial dry weight, increased with the increase of both the activity of AOX and the rate of CN-insensitive and SHAM-sensitive respiration. On the other hand, when WU-2223L was cultivated with SHAM, an inhibitor of AOX, the CN-insensitive and SHAM-sensitive respiration was not detected and the citric acid production and the productivity drastically decreased, although mycelial growth was not affected. These results clearly indicated that the CN-insensitive and SHAM-sensitive respiration catalyzed by AOX, localized in the mitochondria, contributed to citric acid production by A. niger.

    KW - Alternative oxidase

    KW - Aspergillus niger

    KW - Citric acid

    KW - Cyanide-insensitive respiration

    KW - Mitochondria

    UR - http://www.scopus.com/inward/record.url?scp=0034295589&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0034295589&partnerID=8YFLogxK

    M3 - Article

    C2 - 11129572

    AN - SCOPUS:0034295589

    VL - 64

    SP - 2034

    EP - 2039

    JO - Bioscience, Biotechnology and Biochemistry

    JF - Bioscience, Biotechnology and Biochemistry

    SN - 0916-8451

    IS - 10

    ER -