Contribution of histone N-terminal tails to the structure and stability of nucleosomes

Wakana Iwasaki, Yuta Miya, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroaki Tachiwana, Takehiko Shibata, Wataru Kagawa, Hitoshi Kurumizaka

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Histones are the protein components of the nucleosome, which forms the basic architecture of eukaryotic chromatin. Histones H2A, H2B, H3, and H4 are composed of two common regions, the "histone fold" and the "histone tail". Many efforts have been focused on the mechanisms by which the post-translational modifications of histone tails regulate the higher-order chromatin architecture. On the other hand, previous biochemical studies have suggested that histone tails also affect the structure and stability of the nucleosome core particle itself. However, the precise contributions of each histone tail are unclear. In the present study, we determined the crystal structures of four mutant nucleosomes, in which one of the four histones, H2A, H2B, H3, or H4, lacked the N-terminal tail. We found that the deletion of the H2B or H3 N-terminal tail affected histone-DNA interactions and substantially decreased nucleosome stability. These findings provide important information for understanding the complex roles of histone tails in regulating chromatin structure.

Original languageEnglish
Pages (from-to)363-369
Number of pages7
JournalFEBS Open Bio
Volume3
DOIs
Publication statusPublished - 2013 Aug

Fingerprint

Nucleosomes
Histones
Chromatin
Post Translational Protein Processing
Crystal structure

Keywords

  • Chromatin
  • Crystal structure
  • Histone tail
  • Nucleosome
  • Thermal stability assay

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Iwasaki, W., Miya, Y., Horikoshi, N., Osakabe, A., Taguchi, H., Tachiwana, H., ... Kurumizaka, H. (2013). Contribution of histone N-terminal tails to the structure and stability of nucleosomes. FEBS Open Bio, 3, 363-369. https://doi.org/10.1016/j.fob.2013.08.007

Contribution of histone N-terminal tails to the structure and stability of nucleosomes. / Iwasaki, Wakana; Miya, Yuta; Horikoshi, Naoki; Osakabe, Akihisa; Taguchi, Hiroyuki; Tachiwana, Hiroaki; Shibata, Takehiko; Kagawa, Wataru; Kurumizaka, Hitoshi.

In: FEBS Open Bio, Vol. 3, 08.2013, p. 363-369.

Research output: Contribution to journalArticle

Iwasaki, W, Miya, Y, Horikoshi, N, Osakabe, A, Taguchi, H, Tachiwana, H, Shibata, T, Kagawa, W & Kurumizaka, H 2013, 'Contribution of histone N-terminal tails to the structure and stability of nucleosomes', FEBS Open Bio, vol. 3, pp. 363-369. https://doi.org/10.1016/j.fob.2013.08.007
Iwasaki W, Miya Y, Horikoshi N, Osakabe A, Taguchi H, Tachiwana H et al. Contribution of histone N-terminal tails to the structure and stability of nucleosomes. FEBS Open Bio. 2013 Aug;3:363-369. https://doi.org/10.1016/j.fob.2013.08.007
Iwasaki, Wakana ; Miya, Yuta ; Horikoshi, Naoki ; Osakabe, Akihisa ; Taguchi, Hiroyuki ; Tachiwana, Hiroaki ; Shibata, Takehiko ; Kagawa, Wataru ; Kurumizaka, Hitoshi. / Contribution of histone N-terminal tails to the structure and stability of nucleosomes. In: FEBS Open Bio. 2013 ; Vol. 3. pp. 363-369.
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