Abstract
A mutant of Chlamydomonas reinhardtii designated as hf-9 is impaired in fatty acid desaturation of chloroplasts, and showed lowered unsaturation levels of chloroplast lipids, as compared with the parent. The effects of temperature on photosynthesis were compared between hf-9 and the parent for investigation whether or not unsaturation levels of chloroplast lipids are correlated with the thermal properties of photosynthesis. Growth rates determined by turbidity were higher in the parent than in hf-9 at both 10 and 24°C, while similar for the parent and hf-9 at 39°C. The cells grown at 24°C revealed that both activities of CO2-dependent oxygen evolution acid photosystem II were higher in the parent than in hf-9 in the range between 7 and 40°C. In contrast, hf-9 surpassed the parent in both activities at 45°C. Optimal temperatures for both activities were at around 35°C and 40°C in the parent and hf-9, respectively. Incubation of the cells at 41 and 45°C demonstrated that the activity of photosystem II in hf-9 was more tolerant to the high temperatures than that in the parent. These results suggest that lowered unsaturation levels of chloroplast lipids contributed to high temperature tolerance of photosystem II, and eventually to that of photosynthesis.
| Original language | English |
|---|---|
| Pages (from-to) | 333-337 |
| Number of pages | 5 |
| Journal | Journal of Photochemistry and Photobiology B: Biology |
| Volume | 36 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1996 Dec |
| Externally published | Yes |
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Keywords
- Chlamydomonas reinhardtii
- Chloroplast lipids
- Desaturation
- High temperature tolerance
- Photosystem II
ASJC Scopus subject areas
- Plant Science
- Bioengineering
- Physical and Theoretical Chemistry
Cite this
Contribution of lowered unsaturation levels of chloroplast lipids to high temperature tolerance of photosynthesis in Chlamydomonas reinhardtii. / Sato, Norihiro; Sonoike, Kintake; Kawaguchi, Akihiko; Tsuzuki, Mikio.
In: Journal of Photochemistry and Photobiology B: Biology, Vol. 36, No. 3, 12.1996, p. 333-337.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Contribution of lowered unsaturation levels of chloroplast lipids to high temperature tolerance of photosynthesis in Chlamydomonas reinhardtii
AU - Sato, Norihiro
AU - Sonoike, Kintake
AU - Kawaguchi, Akihiko
AU - Tsuzuki, Mikio
PY - 1996/12
Y1 - 1996/12
N2 - A mutant of Chlamydomonas reinhardtii designated as hf-9 is impaired in fatty acid desaturation of chloroplasts, and showed lowered unsaturation levels of chloroplast lipids, as compared with the parent. The effects of temperature on photosynthesis were compared between hf-9 and the parent for investigation whether or not unsaturation levels of chloroplast lipids are correlated with the thermal properties of photosynthesis. Growth rates determined by turbidity were higher in the parent than in hf-9 at both 10 and 24°C, while similar for the parent and hf-9 at 39°C. The cells grown at 24°C revealed that both activities of CO2-dependent oxygen evolution acid photosystem II were higher in the parent than in hf-9 in the range between 7 and 40°C. In contrast, hf-9 surpassed the parent in both activities at 45°C. Optimal temperatures for both activities were at around 35°C and 40°C in the parent and hf-9, respectively. Incubation of the cells at 41 and 45°C demonstrated that the activity of photosystem II in hf-9 was more tolerant to the high temperatures than that in the parent. These results suggest that lowered unsaturation levels of chloroplast lipids contributed to high temperature tolerance of photosystem II, and eventually to that of photosynthesis.
AB - A mutant of Chlamydomonas reinhardtii designated as hf-9 is impaired in fatty acid desaturation of chloroplasts, and showed lowered unsaturation levels of chloroplast lipids, as compared with the parent. The effects of temperature on photosynthesis were compared between hf-9 and the parent for investigation whether or not unsaturation levels of chloroplast lipids are correlated with the thermal properties of photosynthesis. Growth rates determined by turbidity were higher in the parent than in hf-9 at both 10 and 24°C, while similar for the parent and hf-9 at 39°C. The cells grown at 24°C revealed that both activities of CO2-dependent oxygen evolution acid photosystem II were higher in the parent than in hf-9 in the range between 7 and 40°C. In contrast, hf-9 surpassed the parent in both activities at 45°C. Optimal temperatures for both activities were at around 35°C and 40°C in the parent and hf-9, respectively. Incubation of the cells at 41 and 45°C demonstrated that the activity of photosystem II in hf-9 was more tolerant to the high temperatures than that in the parent. These results suggest that lowered unsaturation levels of chloroplast lipids contributed to high temperature tolerance of photosystem II, and eventually to that of photosynthesis.
KW - Chlamydomonas reinhardtii
KW - Chloroplast lipids
KW - Desaturation
KW - High temperature tolerance
KW - Photosystem II
UR - http://www.scopus.com/inward/record.url?scp=0030512672&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030512672&partnerID=8YFLogxK
U2 - 10.1016/S1011-1344(96)07389-7
DO - 10.1016/S1011-1344(96)07389-7
M3 - Article
AN - SCOPUS:0030512672
VL - 36
SP - 333
EP - 337
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
SN - 1011-1344
IS - 3
ER -