Contribution of nitric oxide synthase to human neutrophil chemiluminescence

Satoru Kudoh, Katsuhiko Suzuki, Mutsuo Yamada, Qiang Liu, Shigeyuki Nakaji, Kazuo Sugawara

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A chemiluminescence (CL) assay has been used to measure the reactive oxygen species (ROS)-generating capacity of phagocytes. Primed neutrophils produce ROS and nitric oxide (NO) upon induction of nitric oxide synthase (NOS) activity. NO and superoxide (O2-) form peroxynitrite (ONOO-), and emit CL. We examined the involvement of NOS in the CL response of neutrophils using a method based on the modulation of enzyme activity of NOS by the substrate L-arginine and an inhibitor; L-NAME. We used lipopolysaccharide (LPS) as the neutrophil-priming agent. Addition of sodium azide (NaN3) with horseradish peroxidase (HRP) to luminol-dependent CL, gave a CL response that was significantly enhanced when 10 mmol/L L-arginine was present (p <0.05). suggesting that NOS activity contributed to the CL response of human neutrophils. LPS-primed luminol-dependent CL was significantly inhibited by L-NAME compared with D-NAME. The proportion of the difference between the two inhibitors in luminol-dependent CL was 12.3 ± 15.0%. Therefore, approximately 12% of the LPS-primed luminol-dependent CL decrease induced by L-NAME indicated the contribution of NOS activity to the CL response.

Original languageEnglish
Pages (from-to)335-339
Number of pages5
JournalLuminescence
Volume14
Issue number6
DOIs
Publication statusPublished - 1999 Jan 1

Keywords

  • Chemiluminescence
  • L-NAME
  • Lipopolysaccharide
  • Neutrophils
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Biophysics
  • Chemistry (miscellaneous)

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