Contribution of rat endothelial progenitor cells on three-dimensional network formation in vitro.

Masaki Koga, Ryo Sudo, Yoshinori Abe, Kimiko Yamamoto, Joji Ando, Mariko Ikeda, Kazuo Tanishita

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Endothelial progenitor cell (EPC) transplantation is a promising treatment option for ischemic diseases, and understanding the mechanism mediating angiogenic effects of EPCs is essential to further improve its effectiveness as well as their application to in vitro tissue engineering. Here we investigated EPC contribution on network formation using an in vitro three-dimensional network model. Bovine microvascular endothelial cells (ECs) were seeded on collagen gel to create a three-dimensional network model. Subsequently, EPCs isolated from rat bone marrow were seeded on top of a confluent endothelial cell (EC) monolayer to create EPC+EC model. In this model, EPCs promoted EC network formation. Quantitative analyses revealed that the total length, number, and depth of networks were significantly enhanced with the addition of EPCs. EPCs tended to localize in networks formed inside collagen gel rather than on a confluent monolayer with increasing experimental duration. In addition, EPCs preferentially distributed near sprout positions in a confluent monolayer. Furthermore, EPC-conditioned medium promoted network formation, and vascular endothelial growth factor was detected in the conditioned medium. Taken together, EPCs contributed to network formation by direct incorporation with on-site growth factor secretion. The angiogenic ability of EPCs offers a possible cell source to reconstruct vascularized tissues in vitro.

Original languageEnglish
Pages (from-to)2727-2739
Number of pages13
JournalTissue engineering. Part A
Volume15
Issue number9
DOIs
Publication statusPublished - 2009 Sep
Externally publishedYes

Fingerprint

Endothelial cells
Rats
Endothelial Cells
Monolayers
Conditioned Culture Medium
Collagen
Gels
erucylphosphocholine
Endothelial Progenitor Cells
In Vitro Techniques
Cell Transplantation
Tissue Engineering
Tissue engineering
Vascular Endothelial Growth Factor A
Intercellular Signaling Peptides and Proteins
Bone
Bone Marrow
Tissue

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Contribution of rat endothelial progenitor cells on three-dimensional network formation in vitro. / Koga, Masaki; Sudo, Ryo; Abe, Yoshinori; Yamamoto, Kimiko; Ando, Joji; Ikeda, Mariko; Tanishita, Kazuo.

In: Tissue engineering. Part A, Vol. 15, No. 9, 09.2009, p. 2727-2739.

Research output: Contribution to journalArticle

Koga, M, Sudo, R, Abe, Y, Yamamoto, K, Ando, J, Ikeda, M & Tanishita, K 2009, 'Contribution of rat endothelial progenitor cells on three-dimensional network formation in vitro.', Tissue engineering. Part A, vol. 15, no. 9, pp. 2727-2739. https://doi.org/10.1089/ten.tea.2008.0417
Koga, Masaki ; Sudo, Ryo ; Abe, Yoshinori ; Yamamoto, Kimiko ; Ando, Joji ; Ikeda, Mariko ; Tanishita, Kazuo. / Contribution of rat endothelial progenitor cells on three-dimensional network formation in vitro. In: Tissue engineering. Part A. 2009 ; Vol. 15, No. 9. pp. 2727-2739.
@article{cf7c272ce93845b7af4fcdd74b285563,
title = "Contribution of rat endothelial progenitor cells on three-dimensional network formation in vitro.",
abstract = "Endothelial progenitor cell (EPC) transplantation is a promising treatment option for ischemic diseases, and understanding the mechanism mediating angiogenic effects of EPCs is essential to further improve its effectiveness as well as their application to in vitro tissue engineering. Here we investigated EPC contribution on network formation using an in vitro three-dimensional network model. Bovine microvascular endothelial cells (ECs) were seeded on collagen gel to create a three-dimensional network model. Subsequently, EPCs isolated from rat bone marrow were seeded on top of a confluent endothelial cell (EC) monolayer to create EPC+EC model. In this model, EPCs promoted EC network formation. Quantitative analyses revealed that the total length, number, and depth of networks were significantly enhanced with the addition of EPCs. EPCs tended to localize in networks formed inside collagen gel rather than on a confluent monolayer with increasing experimental duration. In addition, EPCs preferentially distributed near sprout positions in a confluent monolayer. Furthermore, EPC-conditioned medium promoted network formation, and vascular endothelial growth factor was detected in the conditioned medium. Taken together, EPCs contributed to network formation by direct incorporation with on-site growth factor secretion. The angiogenic ability of EPCs offers a possible cell source to reconstruct vascularized tissues in vitro.",
author = "Masaki Koga and Ryo Sudo and Yoshinori Abe and Kimiko Yamamoto and Joji Ando and Mariko Ikeda and Kazuo Tanishita",
year = "2009",
month = "9",
doi = "10.1089/ten.tea.2008.0417",
language = "English",
volume = "15",
pages = "2727--2739",
journal = "Tissue Engineering - Part A.",
issn = "1937-3341",
publisher = "Mary Ann Liebert Inc.",
number = "9",

}

TY - JOUR

T1 - Contribution of rat endothelial progenitor cells on three-dimensional network formation in vitro.

AU - Koga, Masaki

AU - Sudo, Ryo

AU - Abe, Yoshinori

AU - Yamamoto, Kimiko

AU - Ando, Joji

AU - Ikeda, Mariko

AU - Tanishita, Kazuo

PY - 2009/9

Y1 - 2009/9

N2 - Endothelial progenitor cell (EPC) transplantation is a promising treatment option for ischemic diseases, and understanding the mechanism mediating angiogenic effects of EPCs is essential to further improve its effectiveness as well as their application to in vitro tissue engineering. Here we investigated EPC contribution on network formation using an in vitro three-dimensional network model. Bovine microvascular endothelial cells (ECs) were seeded on collagen gel to create a three-dimensional network model. Subsequently, EPCs isolated from rat bone marrow were seeded on top of a confluent endothelial cell (EC) monolayer to create EPC+EC model. In this model, EPCs promoted EC network formation. Quantitative analyses revealed that the total length, number, and depth of networks were significantly enhanced with the addition of EPCs. EPCs tended to localize in networks formed inside collagen gel rather than on a confluent monolayer with increasing experimental duration. In addition, EPCs preferentially distributed near sprout positions in a confluent monolayer. Furthermore, EPC-conditioned medium promoted network formation, and vascular endothelial growth factor was detected in the conditioned medium. Taken together, EPCs contributed to network formation by direct incorporation with on-site growth factor secretion. The angiogenic ability of EPCs offers a possible cell source to reconstruct vascularized tissues in vitro.

AB - Endothelial progenitor cell (EPC) transplantation is a promising treatment option for ischemic diseases, and understanding the mechanism mediating angiogenic effects of EPCs is essential to further improve its effectiveness as well as their application to in vitro tissue engineering. Here we investigated EPC contribution on network formation using an in vitro three-dimensional network model. Bovine microvascular endothelial cells (ECs) were seeded on collagen gel to create a three-dimensional network model. Subsequently, EPCs isolated from rat bone marrow were seeded on top of a confluent endothelial cell (EC) monolayer to create EPC+EC model. In this model, EPCs promoted EC network formation. Quantitative analyses revealed that the total length, number, and depth of networks were significantly enhanced with the addition of EPCs. EPCs tended to localize in networks formed inside collagen gel rather than on a confluent monolayer with increasing experimental duration. In addition, EPCs preferentially distributed near sprout positions in a confluent monolayer. Furthermore, EPC-conditioned medium promoted network formation, and vascular endothelial growth factor was detected in the conditioned medium. Taken together, EPCs contributed to network formation by direct incorporation with on-site growth factor secretion. The angiogenic ability of EPCs offers a possible cell source to reconstruct vascularized tissues in vitro.

UR - http://www.scopus.com/inward/record.url?scp=70350514249&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350514249&partnerID=8YFLogxK

U2 - 10.1089/ten.tea.2008.0417

DO - 10.1089/ten.tea.2008.0417

M3 - Article

VL - 15

SP - 2727

EP - 2739

JO - Tissue Engineering - Part A.

JF - Tissue Engineering - Part A.

SN - 1937-3341

IS - 9

ER -