Contributions of phosphate to DNA adsorption/desorption behaviors on aminosilane-modified magnetic nanoparticles

Tsuyoshi Tanaka, Ririko Sakai, Ryosuke Kobayashi, Keiichi Hatakeyama, Tadashi Matsunaga

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

The adsorption and desorption behaviors of DNA on aminosilane-modified magnetic nanoparticles were investigated by altering both type of anions and solvation state to achieve eficient recovery of DNA useful for subsequent polymerase chain reaction (PCR) analysis. The effects of multiple anions in accordance with the Hofmeister ion series were determined to clarify the contribution of phosphate ions on the effective desorption of DNA from aminosilane surfaces. Efficient DNA desorption (85% recovery) occurred in the presence of 1 M phosphate buffer, however, little DNA desorption was observed using any other anions. This phenomenon indicates that desorption originated from the replacement of DNA by phosphate ions. Furthermore, the adsorption and desorption were significantly affected by the addition of both protic and aprotic solvents. Efficient recovery of adsorbed DNA was attained using deoxynucleotide triphosphates (dNTPs) in place of phosphate buffer and was suitable for subsequent PCR analyses. Therefore, the DNA adsorption/desorption process proposed in this study will be a promising, novel approach for DNA purification with a high recovery ratio that is suitable for subsequent enzymatic reactions, such as PCR or restriction enzyme digestion.

Original languageEnglish
Pages (from-to)2956-2961
Number of pages6
JournalLangmuir
Volume25
Issue number5
DOIs
Publication statusPublished - 2009 Mar 3
Externally publishedYes

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

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