Control of cell behavior on the polymer functionalized biointerface

Hidenori Otsuka, Tomomi Satomi, Koji Ueno, Akiyoshi Taniguchi, Hisatoshi Kobayashi, Junzo Tanaka, Yukio Nagasaki, Kazunori Kataoka, Yohei Fujita

Research output: Contribution to conferencePaper

Abstract

Micropatterned PEGylated substrates with two-dimensional arrays of plasma-etched circular domains were prepared by coating of poly(ethylene glycol) (PEG) based polymer on the substrate, followed by plasma-etching through a metal mask pattern with circular holes. The PEGylated region on the patterned substrate works to repel proteins, consequently, inhibits cell adhesion. Then the micro-patterning of bovine articular chondrocytes or rat primary hepatocytes hetero-spheroids underlaid with endothelial cells was achieved on the plasma-etched circular domains, exposing the base substrate surface. PEG-g-pyridine copolymer was then synthesized for long-term cell culture, which is expected to have long stability, to overcome deterioration of polymer modified surface at room temperature and atmospheric conditions when single-point adsorption was employed.

Original languageEnglish
Pages5145-5146
Number of pages2
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

Keywords

  • Bio-nanointerface
  • Cell patterning
  • Chondrocyte
  • Hepatocyte
  • Spheroid
  • Tissue engineering

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Otsuka, H., Satomi, T., Ueno, K., Taniguchi, A., Kobayashi, H., Tanaka, J., Nagasaki, Y., Kataoka, K., & Fujita, Y. (2005). Control of cell behavior on the polymer functionalized biointerface. 5145-5146. Paper presented at 54th SPSJ Symposium on Macromolecules, Yamagata, Japan.