Control of cell behavior on the polymer functionalized biointerface

Hidenori Otsuka, Tomomi Satomi, Koji Ueno, Akiyoshi Taniguchi, Hisatoshi Kobayashi, Junzo Tanaka, Yukio Nagasaki, Kazunori Kataoka, Yohei Fujita

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Micropatterned PEGylated substrates with two-dimensional arrays of plasma-etched circular domains were prepared by coating of poly(ethylene glycol) (PEG) based polymer on the substrate, followed by plasma-etching through a metal mask pattern with circular holes. The PEGylated region on the patterned substrate works to repel proteins, consequently, inhibits cell adhesion. Then the micro-patterning of bovine articular chondrocytes or rat primary hepatocytes hetero-spheroids underlaid with endothelial cells was achieved on the plasma-etched circular domains, exposing the base substrate surface. PEG-g-pyridine copolymer was then synthesized for long-term cell culture, which is expected to have long stability, to overcome deterioration of polymer modified surface at room temperature and atmospheric conditions when single-point adsorption was employed.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan
Pages5145-5146
Number of pages2
Volume54
Edition2
Publication statusPublished - 2005
Externally publishedYes
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

Fingerprint

Polyethylene glycols
Polymers
Substrates
Plasmas
Plasma etching
Cell adhesion
Endothelial cells
Cell culture
Pyridine
Deterioration
Rats
Masks
Copolymers
Proteins
Adsorption
Coatings
Metals
Temperature
Hepatocytes

Keywords

  • Bio-nanointerface
  • Cell patterning
  • Chondrocyte
  • Hepatocyte
  • Spheroid
  • Tissue engineering

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Otsuka, H., Satomi, T., Ueno, K., Taniguchi, A., Kobayashi, H., Tanaka, J., ... Fujita, Y. (2005). Control of cell behavior on the polymer functionalized biointerface. In Polymer Preprints, Japan (2 ed., Vol. 54, pp. 5145-5146)

Control of cell behavior on the polymer functionalized biointerface. / Otsuka, Hidenori; Satomi, Tomomi; Ueno, Koji; Taniguchi, Akiyoshi; Kobayashi, Hisatoshi; Tanaka, Junzo; Nagasaki, Yukio; Kataoka, Kazunori; Fujita, Yohei.

Polymer Preprints, Japan. Vol. 54 2. ed. 2005. p. 5145-5146.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Otsuka, H, Satomi, T, Ueno, K, Taniguchi, A, Kobayashi, H, Tanaka, J, Nagasaki, Y, Kataoka, K & Fujita, Y 2005, Control of cell behavior on the polymer functionalized biointerface. in Polymer Preprints, Japan. 2 edn, vol. 54, pp. 5145-5146, 54th SPSJ Symposium on Macromolecules, Yamagata, Japan, 05/9/20.
Otsuka H, Satomi T, Ueno K, Taniguchi A, Kobayashi H, Tanaka J et al. Control of cell behavior on the polymer functionalized biointerface. In Polymer Preprints, Japan. 2 ed. Vol. 54. 2005. p. 5145-5146
Otsuka, Hidenori ; Satomi, Tomomi ; Ueno, Koji ; Taniguchi, Akiyoshi ; Kobayashi, Hisatoshi ; Tanaka, Junzo ; Nagasaki, Yukio ; Kataoka, Kazunori ; Fujita, Yohei. / Control of cell behavior on the polymer functionalized biointerface. Polymer Preprints, Japan. Vol. 54 2. ed. 2005. pp. 5145-5146
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