Control of gene transfer on a DNA-fibronectin-apatite composite layer by the incorporation of carbonate and fluoride ions

Yushin Yazaki, Ayako Oyane, Yu Sogo, Atsuo Ito, Atsushi Yamazaki, Hideo Tsurushima

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Gene transfer techniques are useful tools for controlling cell behavior, such as proliferation and differentiation. We have recently developed an efficient area-specific gene transfer system using a DNA-fibronectin-apatite composite layer (DF-Ap layer). In this system, partial dissolution of the composite layer is likely to be a crucial step for gene transfer. In the present study, layer solubility was adjusted by incorporating various contents of carbonate or fluoride ions into the DF-Ap layer via ionic substitution for the apatite crystals. Carbonate ion incorporation increased the solubility of the DF-Ap layer, thereby increasing the efficiency of gene transfer on the layer. In contrast, the incorporation of fluoride ions decreased the solubility of the DF-Ap layer, thereby decreasing the efficiency and delaying the timing of gene transfer on the layer dose-dependently. The present gene transfer system with controllable efficiency and timing would be useful in tissue engineering applications because cell differentiation can be induced effectively by regulating appropriate gene expression with suitable timing.

    Original languageEnglish
    Pages (from-to)4896-4902
    Number of pages7
    JournalBiomaterials
    Volume32
    Issue number21
    DOIs
    Publication statusPublished - 2011 Jul

    Fingerprint

    Gene transfer
    Apatites
    Carbonates
    Apatite
    Fluorides
    Fibronectins
    DNA
    Ions
    Solubility
    Composite materials
    Genes
    Gene Transfer Techniques
    Tissue Engineering
    Cell Differentiation
    Tissue engineering
    Gene expression
    Dissolution
    Substitution reactions
    Gene Expression
    Crystals

    Keywords

    • Controlled drug release
    • Fibronectin
    • Gene transfer
    • Hydroxyapatite coating
    • Hydroxyapatite composite

    ASJC Scopus subject areas

    • Biomaterials
    • Bioengineering
    • Ceramics and Composites
    • Mechanics of Materials
    • Biophysics

    Cite this

    Control of gene transfer on a DNA-fibronectin-apatite composite layer by the incorporation of carbonate and fluoride ions. / Yazaki, Yushin; Oyane, Ayako; Sogo, Yu; Ito, Atsuo; Yamazaki, Atsushi; Tsurushima, Hideo.

    In: Biomaterials, Vol. 32, No. 21, 07.2011, p. 4896-4902.

    Research output: Contribution to journalArticle

    Yazaki, Yushin ; Oyane, Ayako ; Sogo, Yu ; Ito, Atsuo ; Yamazaki, Atsushi ; Tsurushima, Hideo. / Control of gene transfer on a DNA-fibronectin-apatite composite layer by the incorporation of carbonate and fluoride ions. In: Biomaterials. 2011 ; Vol. 32, No. 21. pp. 4896-4902.
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