Controllable three-dimensional sheath flow with a wide range reynolds number and its application for efficient cell sorting

R. Sekine, T. Sakurai, Donghyun Yoon, R. Iizuka, Tetsushi Sekiguchi, T. Funatsu, Shuichi Shoji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed on-chip sorting systems using sol-gel transition of the thermo-reversible gelation polymer (TGP) as carrier flow controlled by the focused infrared (IR) laser [1]. For efficient sorting, we achieved size and position control of sample core flow in three-dimensional (3D) sheath flow. Stable control was realized under wide range of Reynolds number (Re) from 0.1 to 115 by optimizing channel structures of sample and carriers inlets. We applied this device for sorting system of yeast cells expressing green fluorescent protein (GFP). The yeast cells were successfully separated with accuracy of more than 82.1% and purity of more than 97.5%.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1903-1905
Number of pages3
Volume3
Publication statusPublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA
Duration: 2011 Oct 22011 Oct 6

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CitySeattle, WA
Period11/10/211/10/6

Fingerprint

Sorting
Reynolds number
Cells
Yeast
Core samples
Infrared lasers
Position control
Gelation
Sol-gels
Proteins
Polymers

Keywords

  • 3D focusing
  • On-chip sorting
  • Thermoreversible gelation polymer
  • Wide range re
  • Yeast cells separation

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Sekine, R., Sakurai, T., Yoon, D., Iizuka, R., Sekiguchi, T., Funatsu, T., & Shoji, S. (2011). Controllable three-dimensional sheath flow with a wide range reynolds number and its application for efficient cell sorting. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 3, pp. 1903-1905)

Controllable three-dimensional sheath flow with a wide range reynolds number and its application for efficient cell sorting. / Sekine, R.; Sakurai, T.; Yoon, Donghyun; Iizuka, R.; Sekiguchi, Tetsushi; Funatsu, T.; Shoji, Shuichi.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3 2011. p. 1903-1905.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sekine, R, Sakurai, T, Yoon, D, Iizuka, R, Sekiguchi, T, Funatsu, T & Shoji, S 2011, Controllable three-dimensional sheath flow with a wide range reynolds number and its application for efficient cell sorting. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 3, pp. 1903-1905, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, 11/10/2.
Sekine R, Sakurai T, Yoon D, Iizuka R, Sekiguchi T, Funatsu T et al. Controllable three-dimensional sheath flow with a wide range reynolds number and its application for efficient cell sorting. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3. 2011. p. 1903-1905
Sekine, R. ; Sakurai, T. ; Yoon, Donghyun ; Iizuka, R. ; Sekiguchi, Tetsushi ; Funatsu, T. ; Shoji, Shuichi. / Controllable three-dimensional sheath flow with a wide range reynolds number and its application for efficient cell sorting. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3 2011. pp. 1903-1905
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