Controlled formation of magnetite crystal by partial oxidation of ferrous hydroxide in the presence of recombinant magnetotactic bacterial protein Mms6

Yosuke Amemiya, Atsushi Arakaki, Sarah S. Staniland, Tsuyoshi Tanaka, Tadashi Matsunaga

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

Mms6 is a small acidic protein that is tightly associated with bacterial magnetite in Magnetospirillum magneticum AMB-1. This protein has previously shown iron binding activity, allowing it to generate uniform magnetic crystals by co-precipitation of ferrous and ferric ions. Here, magnetite crystals were formed by the partial oxidation of ferrous hydroxide in the presence and absence of Mms6. The crystals synthesised were systematically characterised according to their sizes and morphologies using high-resolution transmission electron microscopy. Mms6-mediated synthesis of magnetite by this methods produced crystals of a uniform size and narrow size distribution with a cubo-octahedral morphology, similar to bacterial magnetite observed in M. magneticum AMB-1. The crystals formed in the absence of Mms6 were octahedral, larger with an increased size distribution. Protein quantification analysis of Mms6 in the synthesised particles indicated tight association of this protein onto the crystal. Furthermore, high affinities to iron ions and a highly charged electrostatic quality suggest that the protein acts as a template for the nucleus formation and/or acts as a growth regulator by recognising crystal faces. The method introduced in this study presents an alternative route for controlling the size and shape of magnetite crystals without the use of organic solvent and high temperatures.

Original languageEnglish
Pages (from-to)5381-5389
Number of pages9
JournalBiomaterials
Volume28
Issue number35
DOIs
Publication statusPublished - 2007 Dec
Externally publishedYes

Fingerprint

Ferrosoferric Oxide
Bacterial Proteins
Magnetite
Oxidation
Crystals
Proteins
Magnetospirillum
Iron
Ions
Static Electricity
Transmission Electron Microscopy
hydroxide ion
Coprecipitation
High resolution transmission electron microscopy
Temperature
Organic solvents
Electrostatics
Growth
Association reactions

Keywords

  • Biomimetic material
  • Biomineralisation
  • Crystal growth
  • Magnetite
  • Magnetotactic bacteria
  • Nanoparticle

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Controlled formation of magnetite crystal by partial oxidation of ferrous hydroxide in the presence of recombinant magnetotactic bacterial protein Mms6. / Amemiya, Yosuke; Arakaki, Atsushi; Staniland, Sarah S.; Tanaka, Tsuyoshi; Matsunaga, Tadashi.

In: Biomaterials, Vol. 28, No. 35, 12.2007, p. 5381-5389.

Research output: Contribution to journalArticle

Amemiya, Yosuke ; Arakaki, Atsushi ; Staniland, Sarah S. ; Tanaka, Tsuyoshi ; Matsunaga, Tadashi. / Controlled formation of magnetite crystal by partial oxidation of ferrous hydroxide in the presence of recombinant magnetotactic bacterial protein Mms6. In: Biomaterials. 2007 ; Vol. 28, No. 35. pp. 5381-5389.
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