Controlling shape and position of vascular formation in engineered tissues by arbitrary assembly of endothelial cells

Hiroaki Takehara, Katsuhisa Sakaguchi, Masatoshi Kuroda, Megumi Muraoka, Kazuyoshi Itoga, Teruo Okano, Tatsuya Shimizu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Cellular self-assembly based on cell-to-cell communication is a well-known tissue organizing process in living bodies. Hence, integrating cellular self-assembly processes into tissue engineering is a promising approach to fabricate well-organized functional tissues. In this research, we investigated the capability of endothelial cells (ECs) to control shape and position of vascular formation using arbitral-assembling techniques in three-dimensional engineered tissues. To quantify the degree of migration of ECs in endothelial network formation, image correlation analysis was conducted. Positive correlation between the original positions of arbitrarily assembled ECs and the positions of formed endothelial networks indicated the potential for controlling shape and position of vascular formations in engineered tissues. To demonstrate the feasibility of controlling vascular formations, engineered tissues with vascular networks in triangle and circle patterns were made. The technique reported here employs cellular self-assembly for tissue engineering and is expected to provide fundamental beneficial methods to supply various functional tissues for drug screening and regenerative medicine.

Original languageEnglish
Article number045006
JournalBiofabrication
Volume7
Issue number4
DOIs
Publication statusPublished - 2015 Nov 6

Fingerprint

Endothelial cells
Blood Vessels
Endothelial Cells
Tissue
Self assembly
Tissue Engineering
Tissue engineering
Preclinical Drug Evaluations
Regenerative Medicine
Process engineering
Cell Communication
Screening
Image processing
Communication
Research
Pharmaceutical Preparations

Keywords

  • cellular self-assembly
  • endothelial cells
  • image correlation analysis
  • three-dimensional tissue

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering

Cite this

Controlling shape and position of vascular formation in engineered tissues by arbitrary assembly of endothelial cells. / Takehara, Hiroaki; Sakaguchi, Katsuhisa; Kuroda, Masatoshi; Muraoka, Megumi; Itoga, Kazuyoshi; Okano, Teruo; Shimizu, Tatsuya.

In: Biofabrication, Vol. 7, No. 4, 045006, 06.11.2015.

Research output: Contribution to journalArticle

Takehara, Hiroaki ; Sakaguchi, Katsuhisa ; Kuroda, Masatoshi ; Muraoka, Megumi ; Itoga, Kazuyoshi ; Okano, Teruo ; Shimizu, Tatsuya. / Controlling shape and position of vascular formation in engineered tissues by arbitrary assembly of endothelial cells. In: Biofabrication. 2015 ; Vol. 7, No. 4.
@article{5a5d577926954f9aab8e07e4c397a312,
title = "Controlling shape and position of vascular formation in engineered tissues by arbitrary assembly of endothelial cells",
abstract = "Cellular self-assembly based on cell-to-cell communication is a well-known tissue organizing process in living bodies. Hence, integrating cellular self-assembly processes into tissue engineering is a promising approach to fabricate well-organized functional tissues. In this research, we investigated the capability of endothelial cells (ECs) to control shape and position of vascular formation using arbitral-assembling techniques in three-dimensional engineered tissues. To quantify the degree of migration of ECs in endothelial network formation, image correlation analysis was conducted. Positive correlation between the original positions of arbitrarily assembled ECs and the positions of formed endothelial networks indicated the potential for controlling shape and position of vascular formations in engineered tissues. To demonstrate the feasibility of controlling vascular formations, engineered tissues with vascular networks in triangle and circle patterns were made. The technique reported here employs cellular self-assembly for tissue engineering and is expected to provide fundamental beneficial methods to supply various functional tissues for drug screening and regenerative medicine.",
keywords = "cellular self-assembly, endothelial cells, image correlation analysis, three-dimensional tissue",
author = "Hiroaki Takehara and Katsuhisa Sakaguchi and Masatoshi Kuroda and Megumi Muraoka and Kazuyoshi Itoga and Teruo Okano and Tatsuya Shimizu",
year = "2015",
month = "11",
day = "6",
doi = "10.1088/1758-5090/7/4/045006",
language = "English",
volume = "7",
journal = "Biofabrication",
issn = "1758-5082",
publisher = "IOP Publishing Ltd.",
number = "4",

}

TY - JOUR

T1 - Controlling shape and position of vascular formation in engineered tissues by arbitrary assembly of endothelial cells

AU - Takehara, Hiroaki

AU - Sakaguchi, Katsuhisa

AU - Kuroda, Masatoshi

AU - Muraoka, Megumi

AU - Itoga, Kazuyoshi

AU - Okano, Teruo

AU - Shimizu, Tatsuya

PY - 2015/11/6

Y1 - 2015/11/6

N2 - Cellular self-assembly based on cell-to-cell communication is a well-known tissue organizing process in living bodies. Hence, integrating cellular self-assembly processes into tissue engineering is a promising approach to fabricate well-organized functional tissues. In this research, we investigated the capability of endothelial cells (ECs) to control shape and position of vascular formation using arbitral-assembling techniques in three-dimensional engineered tissues. To quantify the degree of migration of ECs in endothelial network formation, image correlation analysis was conducted. Positive correlation between the original positions of arbitrarily assembled ECs and the positions of formed endothelial networks indicated the potential for controlling shape and position of vascular formations in engineered tissues. To demonstrate the feasibility of controlling vascular formations, engineered tissues with vascular networks in triangle and circle patterns were made. The technique reported here employs cellular self-assembly for tissue engineering and is expected to provide fundamental beneficial methods to supply various functional tissues for drug screening and regenerative medicine.

AB - Cellular self-assembly based on cell-to-cell communication is a well-known tissue organizing process in living bodies. Hence, integrating cellular self-assembly processes into tissue engineering is a promising approach to fabricate well-organized functional tissues. In this research, we investigated the capability of endothelial cells (ECs) to control shape and position of vascular formation using arbitral-assembling techniques in three-dimensional engineered tissues. To quantify the degree of migration of ECs in endothelial network formation, image correlation analysis was conducted. Positive correlation between the original positions of arbitrarily assembled ECs and the positions of formed endothelial networks indicated the potential for controlling shape and position of vascular formations in engineered tissues. To demonstrate the feasibility of controlling vascular formations, engineered tissues with vascular networks in triangle and circle patterns were made. The technique reported here employs cellular self-assembly for tissue engineering and is expected to provide fundamental beneficial methods to supply various functional tissues for drug screening and regenerative medicine.

KW - cellular self-assembly

KW - endothelial cells

KW - image correlation analysis

KW - three-dimensional tissue

UR - http://www.scopus.com/inward/record.url?scp=84954097764&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84954097764&partnerID=8YFLogxK

U2 - 10.1088/1758-5090/7/4/045006

DO - 10.1088/1758-5090/7/4/045006

M3 - Article

C2 - 26545138

AN - SCOPUS:84954097764

VL - 7

JO - Biofabrication

JF - Biofabrication

SN - 1758-5082

IS - 4

M1 - 045006

ER -