Convenient method to purify hemoglobin

Hiromi Sakai, Shinji Takeoka, Hiroyuki Nishide, Eishun Tsuchida

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


A convenient method to purify Hb solution from outdated RBC has been established for the starting material of Hb-based blood substitutes. To prevent MetHb formation during the procedure, Hb in RBC was carbonylated in advance. Then RBC was mixed with organic solvent for hemolysis and centrifuged for removal of stroma. The resulting SFHb solution was heated at 60C and generated precipitates were removed out by centrifugation. The purity of Hb (25 g/dl) was confirmed by SDS-PAGE. IEF and oxygen binding property of the Hb solution also guaranteed its purity and no denaturation of Hb. This method is applicable to large scale production of the purified Hb for the starting material of Hb-based blood substitutes.

Original languageEnglish
Pages (from-to)651-656
Number of pages6
JournalArtificial Cells, Blood Substitutes, and Biotechnology
Issue number3
Publication statusPublished - 1994 Jan

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering


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