Convenient method to purify hemoglobin

H. Sakai, Shinji Takeoka, Hiroyuki Nishide, E. Tsuchida

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A convenient method to purify Hb solution from outdated RBC has been established for the starting material of Hb-based blood substitutes. To prevent MetHb formation during the procedure, Hb in RBC was carbonylated in advance. Then RBC was mixed with organic solvent for hemolysis and centrifuged for removal of stroma. The resulting SFHb solution was heated at 60°C and generated precipitates were removed out by centrifugation. The purity of Hb (25 g/dl) was confirmed by SDS-PAGE. IEF and oxygen binding property of the Hb solution also guaranteed its purity and no denaturation of Hb. This method is applicable to large scale production of the purified Hb for the starting material of Hb-based blood substitutes.

Original languageEnglish
Pages (from-to)651-656
Number of pages6
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume22
Issue number3
Publication statusPublished - 1994

Fingerprint

Hemoglobin
Blood substitutes
Blood Substitutes
Hemoglobins
Denaturation
Centrifugation
Hemolysis
Organic solvents
Precipitates
Polyacrylamide Gel Electrophoresis
Oxygen

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Biomaterials
  • Hematology

Cite this

Convenient method to purify hemoglobin. / Sakai, H.; Takeoka, Shinji; Nishide, Hiroyuki; Tsuchida, E.

In: Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, Vol. 22, No. 3, 1994, p. 651-656.

Research output: Contribution to journalArticle

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