Alanine was the best amino donor among various amino acids and NH4Cl for the phenylalanine production of Micrococus luteus. l-Alanine was regenerated at the rate of 9.2μ moles/min/g dry cells from NH4Cl and pyruvate by immobilized Clostridium butyricum-alanine dehydrogenase. l-Phenylalanine was continuously produced from hydrogen, NH4Cl and phenylpyruvate by coupling immobilized C. butyricum, alanine dehydrogenase and M. luteus. The rate of phenylalanine production was 1.74 μ moles/min/g dry cells.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology