Cooperativity between two heads of Dictyostelium myosin II in in vitro motility and ATP hydrolysis

Kohji Ito, Xiong Liu, Eisaku Katayama, Taro Q.P. Uyeda

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

To elucidate the significance of the two-headed structure of myosin II, we have engineered and characterized recombinant single-headed myosin II. A tail segment of a myosin II heavy chain fused with a His-tag was expressed in wild-type Dictyostelium cells. Single-headed myosin, which consists of a full length myosin heavy chain and a tagged tail, was isolated on the basis of the affinities for Nickel agarose and actin. Actin sliding velocity by the single-headed myosin was about half of the two-headed, whereas the minimum density of the heads to support continuous movement was twofold higher. Actin-activated MgATPase activity of the single-headed myosin in solution in the presence of 24 μM actin was less than half of the two headed. This decrease is primarily because of fourfold-elevated Kapp for actin and secondary to 40% lower Vmax. These results suggest that the two heads of a Dictyostelium myosin II molecule act cooperatively on an actin filament. We propose a mechanism by which two heads move actin efficiently based on the cooperativity.

Original languageEnglish
Pages (from-to)985-992
Number of pages8
JournalBiophysical Journal
Volume76
Issue number2
DOIs
Publication statusPublished - 1999
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics

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