Coprecipitation of cytochrome C with calcium phosphate on hydroxyapatite ceramic

Yu Sogo, Atsuo Ito, Koshiro Fukasawa, Nao Kondo, Yusuke Ishikawa, Noboru Ichinose, Atsushi Yamazaki

    Research output: Contribution to journalArticle

    28 Citations (Scopus)

    Abstract

    Cytochrome C (cyt C), which has a molecular weight and an isoelectric point similar to those of fibroblast growth factor (FGF-2), was coprecipitated with calcium phosphates in the presence or absence of a hydroxyapatite ceramic (HAP) using supersaturated calcium phosphate solutions that can be prepared by mixing infusion fluids. The precipitates formed in the solution consisted of amorphous calcium phosphate in the absence of a HAP. In the presence of a HAP, precipitates morphologically identical to bone-like apatite formed a calcium phosphate layer on the surface of HAP. The optimum conditions for coprecipitation of cyt C with calcium phosphate on HAP were a Ca/P molar ratio of 1.5 and a NaHCO3 concentration of 7.90 mM. The release of cyt C from the calcium phosphate layer continued for at least 10 days in the physiological salt solution. The results suggest that FGF-2 is also expected to be coprecipitated with calcium phosphates onto HAPs using infusion fluids.

    Original languageEnglish
    Pages (from-to)526-530
    Number of pages5
    JournalCurrent Applied Physics
    Volume5
    Issue number5
    DOIs
    Publication statusPublished - 2005 Jul

    Fingerprint

    calcium phosphates
    cytochromes
    Calcium phosphate
    Durapatite
    Cytochromes
    Coprecipitation
    Hydroxyapatite
    ceramics
    Proteins
    Calcium Phosphates
    Fibroblast Growth Factor 2
    Precipitates
    Apatites
    precipitates
    Fluids
    Fibroblast Growth Factors
    Bone
    fluids
    Apatite
    fibroblasts

    Keywords

    • ACP
    • Coprecipitation
    • Cytochrome C
    • Fibroblast growth factor

    ASJC Scopus subject areas

    • Condensed Matter Physics
    • Surfaces and Interfaces
    • Materials Science (miscellaneous)

    Cite this

    Coprecipitation of cytochrome C with calcium phosphate on hydroxyapatite ceramic. / Sogo, Yu; Ito, Atsuo; Fukasawa, Koshiro; Kondo, Nao; Ishikawa, Yusuke; Ichinose, Noboru; Yamazaki, Atsushi.

    In: Current Applied Physics, Vol. 5, No. 5, 07.2005, p. 526-530.

    Research output: Contribution to journalArticle

    Sogo, Yu ; Ito, Atsuo ; Fukasawa, Koshiro ; Kondo, Nao ; Ishikawa, Yusuke ; Ichinose, Noboru ; Yamazaki, Atsushi. / Coprecipitation of cytochrome C with calcium phosphate on hydroxyapatite ceramic. In: Current Applied Physics. 2005 ; Vol. 5, No. 5. pp. 526-530.
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    abstract = "Cytochrome C (cyt C), which has a molecular weight and an isoelectric point similar to those of fibroblast growth factor (FGF-2), was coprecipitated with calcium phosphates in the presence or absence of a hydroxyapatite ceramic (HAP) using supersaturated calcium phosphate solutions that can be prepared by mixing infusion fluids. The precipitates formed in the solution consisted of amorphous calcium phosphate in the absence of a HAP. In the presence of a HAP, precipitates morphologically identical to bone-like apatite formed a calcium phosphate layer on the surface of HAP. The optimum conditions for coprecipitation of cyt C with calcium phosphate on HAP were a Ca/P molar ratio of 1.5 and a NaHCO3 concentration of 7.90 mM. The release of cyt C from the calcium phosphate layer continued for at least 10 days in the physiological salt solution. The results suggest that FGF-2 is also expected to be coprecipitated with calcium phosphates onto HAPs using infusion fluids.",
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    AU - Ito, Atsuo

    AU - Fukasawa, Koshiro

    AU - Kondo, Nao

    AU - Ishikawa, Yusuke

    AU - Ichinose, Noboru

    AU - Yamazaki, Atsushi

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