Coprecipitation of DNA-lipid complexes with apatite and comparison with superficial adsorption for gene transfer applications

Yushin Yazaki, Ayako Oyane, Hideo Tsurushima, Hiroko Araki, Yu Sogo, Atsuo Ito, Atsushi Yamazaki

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Apatite can mediate gene transfer into cells by serving as a safe and biocompatible immobilization matrix for DNA and transfection reagents. Recently, an apatite layer that immobilized DNA-lipid complexes was prepared by a coprecipitation process in a supersaturated calcium phosphate solution. This composite layer (DNA-lipid-apatite layer) showed a higher gene transfer capability than an apatite layer with superficially adsorbed DNA-lipid complexes (DNA-lipid-adsorbed apatite layer). In this study, the DNA-lipid-apatite layer and the DNA-lipid-adsorbed apatite layer were compared for their physicochemical properties and gene transfer capabilities. The higher gene transfer capability of the DNA-lipid-apatite layer compared with that of the DNA-lipid-adsorbed apatite layer was reconfirmed by a luciferase assay using epithelial-like CHO-K1 cells. Physicochemical structure analyses showed that the DNA-lipid-apatite layer possessed a larger capacity for DNA-lipid complexes than the DNA-lipid-adsorbed apatite layer. The DNA-lipid-apatite layer released DNA-lipid complexes in a slow and sustained manner, whereas the DNA-lipid-adsorbed apatite layer released them in short bursts. Consequently, the release of DNA-lipid complexes from the DNA-lipid-apatite layer was larger in amount and longer in duration than release from the DNA-lipid-adsorbed apatite layer. This difference in release profiles may be responsible for the higher gene transfer capability of the DNA-lipid-apatite layer compared with that of the DNA-lipid-adsorbed apatite layer. The coprecipitation process and the resulting DNA-lipid-apatite layer have many applications in tissue engineering.

    Original languageEnglish
    Pages (from-to)937-945
    Number of pages9
    JournalJournal of Biomaterials Applications
    Volume28
    Issue number6
    DOIs
    Publication statusPublished - 2014 Feb

    Fingerprint

    Gene transfer
    Apatites
    Apatite
    Coprecipitation
    Lipids
    DNA
    Adsorption
    Immobilized Nucleic Acids

    Keywords

    • apatite
    • composite
    • DNA
    • gene transfer
    • Lipid

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Biomaterials

    Cite this

    Coprecipitation of DNA-lipid complexes with apatite and comparison with superficial adsorption for gene transfer applications. / Yazaki, Yushin; Oyane, Ayako; Tsurushima, Hideo; Araki, Hiroko; Sogo, Yu; Ito, Atsuo; Yamazaki, Atsushi.

    In: Journal of Biomaterials Applications, Vol. 28, No. 6, 02.2014, p. 937-945.

    Research output: Contribution to journalArticle

    Yazaki, Yushin ; Oyane, Ayako ; Tsurushima, Hideo ; Araki, Hiroko ; Sogo, Yu ; Ito, Atsuo ; Yamazaki, Atsushi. / Coprecipitation of DNA-lipid complexes with apatite and comparison with superficial adsorption for gene transfer applications. In: Journal of Biomaterials Applications. 2014 ; Vol. 28, No. 6. pp. 937-945.
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