Correlation between astrocyte activity and recovery from blood-brain barrier breakdown caused by brain injury

Hiroko Ikeshima-Kataoka, Masato Yasui

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Glial activation is associated with cell proliferation and upregulation of astrocyte marker expression following traumatic injury in the brain. However, the biological significance of these processes remains unclear. In the present study, astrocyte activation was investigated in a murine brain injury model. Brain injury induces blood-brain barrier (BBB) breakdown and immunoglobulin G (IgG) leak into the brain parenchyma. The recovery of BBB breakdown was evaluated by analyzing immunofluorescent staining with mouse IgG antibody. IgG leakage was greatest at 1 day after stab wound injury and decreased thereafter, and almost diminished after 7 days. Bromodeoxy uridine incorporation was used, and astrocyte proliferation rates were examined by coimmunostaining with anti-bromodeoxy uridine and anti-glial fibrillary acid protein antibodies. Consistent with IgG leakage assays, astrocyte activation was the highest at day 3 and decreased after 7 days. Moreover, in reverse transcriptase-quantitative-PCR experiments, genes associated with BBB integrity were downregulated immediately after BBB breakdown and recovered to basal expression levels within 7 days. These data indicated that astrocyte activation correlated with BBB recovery from breakdown following brain injury.

    Original languageEnglish
    Pages (from-to)894-900
    Number of pages7
    JournalNeuroReport
    Volume27
    Issue number12
    DOIs
    Publication statusPublished - 2016 Aug 17

    Fingerprint

    Blood-Brain Barrier
    Astrocytes
    Brain Injuries
    Immunoglobulin G
    Uridine
    Stab Wounds
    Biological Phenomena
    Antibodies
    Glial Fibrillary Acidic Protein
    Reverse Transcriptase Polymerase Chain Reaction
    Neuroglia
    Up-Regulation
    Down-Regulation
    Cell Proliferation
    Staining and Labeling
    Wounds and Injuries
    Brain
    Genes

    Keywords

    • blood-brain barrier
    • bromodeoxy uridine
    • immunoglobulin G
    • reactive astrocytes
    • stab wound injury

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Correlation between astrocyte activity and recovery from blood-brain barrier breakdown caused by brain injury. / Ikeshima-Kataoka, Hiroko; Yasui, Masato.

    In: NeuroReport, Vol. 27, No. 12, 17.08.2016, p. 894-900.

    Research output: Contribution to journalArticle

    Ikeshima-Kataoka, Hiroko ; Yasui, Masato. / Correlation between astrocyte activity and recovery from blood-brain barrier breakdown caused by brain injury. In: NeuroReport. 2016 ; Vol. 27, No. 12. pp. 894-900.
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