Cortical neurons from intrauterine growth retardation rats exhibit lower response to neurotrophin BDNF

Midori Ninomiya, T. Numakawa Tadahiro, Naoki Adachi, Miyako Furuta, Shuichi Chiba, Misty Richards, Shigenobu Shibata, Hiroshi Kunugi

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    Intrauterine growth retardation (IUGR) is putatively involved in the pathophysiology of schizophrenia. The animal model of IUGR induced by synthetic thromboxane A2 (TXA2) is useful to clarify the effect of IUGR on pups' brains, however, analysis at the cellular level is still needed. Brain-derived neurotrophic factor (BDNF), which plays a role in neuronal survival and synaptic plasticity in the central nervous system (CNS), may also be associated with schizophrenia. However, the possible relationship between IUGR and BDNF function remains unclear. Here, we examined how IUGR by TXA2 impacts BDNF function by using dissociated cortical neurons. We found that, although BDNF levels in cultured neurons from the cerebral cortex of low birth weight pups with IUGR were unchanged, TrkB (BDNF receptor) was decreased compared with control-rats. BDNF-stimulated MAPK/ERK1/2 and PI3K/Akt pathways, which are downstream intracellular signaling pathways of TrkB, were repressed in IUGR-rat cultures. Expression of glutamate receptors such as GluA1 and GluN2A was also suppressed in IUGR-rat cultures. Furthermore, in IUGR-rat cultures, anti-apoptotic protein Bcl2 was decreased and BDNF failed to prevent neurons from cell death caused by serum-deprivation. Taken together, IUGR resulted in reductions in cell viability and in synaptic function following TrkB down-regulation, which may play a role in schizophrenia-like behaviors.

    Original languageEnglish
    Pages (from-to)104-109
    Number of pages6
    JournalNeuroscience Letters
    Volume476
    Issue number2
    DOIs
    Publication statusPublished - 2010 May

    Fingerprint

    Fetal Growth Retardation
    Brain-Derived Neurotrophic Factor
    Nerve Growth Factors
    Neurons
    Schizophrenia
    Thromboxane A2
    Neuronal Plasticity
    trkB Receptor
    Apoptosis Regulatory Proteins
    Glutamate Receptors
    Low Birth Weight Infant
    Phosphatidylinositol 3-Kinases
    Cerebral Cortex
    Cell Survival
    Cell Death
    Down-Regulation
    Central Nervous System
    Animal Models

    Keywords

    • BDNF
    • Cell death
    • Intrauterine growth retardation
    • TrkB

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Ninomiya, M., Numakawa Tadahiro, T., Adachi, N., Furuta, M., Chiba, S., Richards, M., ... Kunugi, H. (2010). Cortical neurons from intrauterine growth retardation rats exhibit lower response to neurotrophin BDNF. Neuroscience Letters, 476(2), 104-109. https://doi.org/10.1016/j.neulet.2010.03.082

    Cortical neurons from intrauterine growth retardation rats exhibit lower response to neurotrophin BDNF. / Ninomiya, Midori; Numakawa Tadahiro, T.; Adachi, Naoki; Furuta, Miyako; Chiba, Shuichi; Richards, Misty; Shibata, Shigenobu; Kunugi, Hiroshi.

    In: Neuroscience Letters, Vol. 476, No. 2, 05.2010, p. 104-109.

    Research output: Contribution to journalArticle

    Ninomiya, M, Numakawa Tadahiro, T, Adachi, N, Furuta, M, Chiba, S, Richards, M, Shibata, S & Kunugi, H 2010, 'Cortical neurons from intrauterine growth retardation rats exhibit lower response to neurotrophin BDNF', Neuroscience Letters, vol. 476, no. 2, pp. 104-109. https://doi.org/10.1016/j.neulet.2010.03.082
    Ninomiya, Midori ; Numakawa Tadahiro, T. ; Adachi, Naoki ; Furuta, Miyako ; Chiba, Shuichi ; Richards, Misty ; Shibata, Shigenobu ; Kunugi, Hiroshi. / Cortical neurons from intrauterine growth retardation rats exhibit lower response to neurotrophin BDNF. In: Neuroscience Letters. 2010 ; Vol. 476, No. 2. pp. 104-109.
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