Abstract
The coexistence of prolactin (PRL) and growth hormone (GH) was previously demonstrated in newly hatched bullfrog (Rana catesbeiana) tadpoles, whereas in adult bullfrogs, there were no cells containing both PRL and GH. However, a cell blot assay with enzymatically dispersed adult pituitary cells demonstrated the existence of cells secreting both PRL and GH. The number of cells secreting both PRL and GH was reduced by a protein synthesis inhibitor, cycloheximide, but not by an RNA synthesis inhibitor, actinomycin D. In situ hybridization and immunostaining of intact pituitary glands revealed the existence of GH mRNA in some of the PRL-immunoreactive cells and of PRL mRNA in some of the GH-immunoreactive cells. We propose that dispersion of the pituitary cells triggered the translation of GH mRNA in the PRL cells and/or of PRL mRNA in the GH cells.
| Original language | English |
|---|---|
| Pages (from-to) | 10-16 |
| Number of pages | 7 |
| Journal | General and Comparative Endocrinology |
| Volume | 122 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2001 |
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Keywords
- Bullfrog
- Cosecretion
- Dispersed pituitary cells
- Growth hormone
- Prolactin
- Rana catesbeiana
ASJC Scopus subject areas
- Endocrinology
Cite this
Cosecretion of prolactin and growth hormone by dispersed pituitary cells of the adult bullfrog, Rana catesbeiana. / Oguchi, Atsuko; Aida, Tomomi; Koda, Aya; Shioda, Seiji; Nakajo, Shigeo; Kobayashi, Tetsuya; Tanaka, Shigeyasu; Yamamoto, Kazutoshi; Kikuyama, Sakaé.
In: General and Comparative Endocrinology, Vol. 122, No. 1, 2001, p. 10-16.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cosecretion of prolactin and growth hormone by dispersed pituitary cells of the adult bullfrog, Rana catesbeiana
AU - Oguchi, Atsuko
AU - Aida, Tomomi
AU - Koda, Aya
AU - Shioda, Seiji
AU - Nakajo, Shigeo
AU - Kobayashi, Tetsuya
AU - Tanaka, Shigeyasu
AU - Yamamoto, Kazutoshi
AU - Kikuyama, Sakaé
PY - 2001
Y1 - 2001
N2 - The coexistence of prolactin (PRL) and growth hormone (GH) was previously demonstrated in newly hatched bullfrog (Rana catesbeiana) tadpoles, whereas in adult bullfrogs, there were no cells containing both PRL and GH. However, a cell blot assay with enzymatically dispersed adult pituitary cells demonstrated the existence of cells secreting both PRL and GH. The number of cells secreting both PRL and GH was reduced by a protein synthesis inhibitor, cycloheximide, but not by an RNA synthesis inhibitor, actinomycin D. In situ hybridization and immunostaining of intact pituitary glands revealed the existence of GH mRNA in some of the PRL-immunoreactive cells and of PRL mRNA in some of the GH-immunoreactive cells. We propose that dispersion of the pituitary cells triggered the translation of GH mRNA in the PRL cells and/or of PRL mRNA in the GH cells.
AB - The coexistence of prolactin (PRL) and growth hormone (GH) was previously demonstrated in newly hatched bullfrog (Rana catesbeiana) tadpoles, whereas in adult bullfrogs, there were no cells containing both PRL and GH. However, a cell blot assay with enzymatically dispersed adult pituitary cells demonstrated the existence of cells secreting both PRL and GH. The number of cells secreting both PRL and GH was reduced by a protein synthesis inhibitor, cycloheximide, but not by an RNA synthesis inhibitor, actinomycin D. In situ hybridization and immunostaining of intact pituitary glands revealed the existence of GH mRNA in some of the PRL-immunoreactive cells and of PRL mRNA in some of the GH-immunoreactive cells. We propose that dispersion of the pituitary cells triggered the translation of GH mRNA in the PRL cells and/or of PRL mRNA in the GH cells.
KW - Bullfrog
KW - Cosecretion
KW - Dispersed pituitary cells
KW - Growth hormone
KW - Prolactin
KW - Rana catesbeiana
UR - http://www.scopus.com/inward/record.url?scp=0035053755&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035053755&partnerID=8YFLogxK
U2 - 10.1006/gcen.2001.7611
DO - 10.1006/gcen.2001.7611
M3 - Article
C2 - 11352548
AN - SCOPUS:0035053755
VL - 122
SP - 10
EP - 16
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
SN - 0016-6480
IS - 1
ER -