CpG methylation of the CENP-B box reduces human CENP-B binding

Yoshinori Tanaka, Hitoshi Kurumizaka, Shigeyuki Yokoyama

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    In eukaryotes, CpG methylation is an epigenetic DNA modification that is important for heterochromatin formation. Centromere protein B (CENP-B) specifically binds to the centromeric 17 base-pair CENP-B box DNA, which contains two CpG dinucleotides. In this study, we tested complex formation by the DNA-binding domain of CENP-B with methylated and unmethylated CENP-B box DNAs, and found that CENP-B preferentially binds to the unmethylated CENP-B box DNA. Competition analyses revealed that the affinity of CENP-B for the CENP-B box DNA is reduced nearly to the level of nonspecific DNA binding by CpG methylation.

    Original languageEnglish
    Pages (from-to)282-289
    Number of pages8
    JournalFEBS Journal
    Volume272
    Issue number1
    DOIs
    Publication statusPublished - 2005 Jan

    Fingerprint

    Centromere Protein B
    Methylation
    Protein Binding
    DNA
    Heterochromatin
    human CENPB protein
    Eukaryota
    Epigenomics
    Base Pairing

    Keywords

    • CENP-B
    • Centromere
    • Chromatin
    • DNA methylation
    • Heterochromatin

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    CpG methylation of the CENP-B box reduces human CENP-B binding. / Tanaka, Yoshinori; Kurumizaka, Hitoshi; Yokoyama, Shigeyuki.

    In: FEBS Journal, Vol. 272, No. 1, 01.2005, p. 282-289.

    Research output: Contribution to journalArticle

    Tanaka, Yoshinori ; Kurumizaka, Hitoshi ; Yokoyama, Shigeyuki. / CpG methylation of the CENP-B box reduces human CENP-B binding. In: FEBS Journal. 2005 ; Vol. 272, No. 1. pp. 282-289.
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