Crassulacean acid metabolism induction in mesembryanthemum crystallinum can be estimated by non-photochemical quenching upon actinic illumination during the Dark Period

Tatsuya Matsuoka, Aya Onozawa, Kintake Sonoike, Shin Kore-Eda

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Mesembryanthemum crystallinum, which switches the mode of photosynthesis from C 3 to crassulacean acid metabolism (CAM) upon high salt stress, was shown here to exhibit diurnal changes in not only the CO 2 fixation pathway but also Chl fluorescence parameters under CAM-induced conditions. We conducted comprehensive time course measurements of M. crystallinum leaf Chl fluorescence using the same leaf throughout the CAM induction period. By doing so, we were able to distinguish the effect of CAM induction from that of photoinhibition and avoid the possible effects of differences in foliar age. We found that the diurnal change in the status of electron transfer could be ascribed to the formation of a proton gradient across thylakoid membranes presumably resulting from diurnal changes in the ATP/ADP ratio reported earlier. The electron transport by actinic illumination thus became limited at the step of plastoquinol oxidation by the Cyt b 6 /f complex in the 'night' period upon CAM induction, resulting in high levels of non-photochemical quenching. The actinically induced non-photochemical quenching in the 'night' period correlated well with the degree of CAM induction. Chl fluorescence parameters, such as NPQ or qN, could be used as a simple indexing system for the CAM induction.

    Original languageEnglish
    Pages (from-to)1966-1975
    Number of pages10
    JournalPlant and Cell Physiology
    Volume59
    Issue number10
    DOIs
    Publication statusPublished - 2018 Oct 1

    Fingerprint

    Mesembryanthemum
    Mesembryanthemum crystallinum
    Crassulacean acid metabolism
    scotophase
    Lighting
    lighting
    Acids
    Fluorescence
    fluorescence
    electron transfer
    Thylakoids
    Photosynthesis
    photoinhibition
    Carbon Monoxide
    Electron Transport
    thylakoids
    Adenosine Diphosphate
    protons
    salt stress
    Protons

    Keywords

    • Chl fluorescence measurements
    • Common ice plant (Mesembryanthemum crystallinum L.)
    • Crassulacean acid metabolism (CAM)
    • Non-photochemical quenching (NPQ)
    • Photoinhibition
    • Salt stress

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

    Cite this

    Crassulacean acid metabolism induction in mesembryanthemum crystallinum can be estimated by non-photochemical quenching upon actinic illumination during the Dark Period. / Matsuoka, Tatsuya; Onozawa, Aya; Sonoike, Kintake; Kore-Eda, Shin.

    In: Plant and Cell Physiology, Vol. 59, No. 10, 01.10.2018, p. 1966-1975.

    Research output: Contribution to journalArticle

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    abstract = "Mesembryanthemum crystallinum, which switches the mode of photosynthesis from C 3 to crassulacean acid metabolism (CAM) upon high salt stress, was shown here to exhibit diurnal changes in not only the CO 2 fixation pathway but also Chl fluorescence parameters under CAM-induced conditions. We conducted comprehensive time course measurements of M. crystallinum leaf Chl fluorescence using the same leaf throughout the CAM induction period. By doing so, we were able to distinguish the effect of CAM induction from that of photoinhibition and avoid the possible effects of differences in foliar age. We found that the diurnal change in the status of electron transfer could be ascribed to the formation of a proton gradient across thylakoid membranes presumably resulting from diurnal changes in the ATP/ADP ratio reported earlier. The electron transport by actinic illumination thus became limited at the step of plastoquinol oxidation by the Cyt b 6 /f complex in the 'night' period upon CAM induction, resulting in high levels of non-photochemical quenching. The actinically induced non-photochemical quenching in the 'night' period correlated well with the degree of CAM induction. Chl fluorescence parameters, such as NPQ or qN, could be used as a simple indexing system for the CAM induction.",
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