Abstract
The pond snail Lymnaea stagnalis is an excellent model system in which to study the neuronal and molecular substrates of associative learning and its consolidation into long-term memory. Until now, the presence of cyclic AMP (cAMP)-responsive element binding protein (CREB), which is believed to be a necessary component in the process of a learned behavior that is consolidated into long-term memory, has only been assumed in Lymnaea neurons. We therefore cloned and analyzed the cDNA sequences of homologues of CREB1 and CREB2 and determined the presence of these mRNAs in identifiable neurons of the central nervous system (CNS) of L. stagnalis. The deduced amino acid sequence of Lymnaea CREB1 is homologous to transcriptional activators, mammalian CREB1 and Aplysia CREB1a, in the C-terminal DNA binding (bZIP) and phosphorylation domains, whereas the deduced amino acid sequence of Lymnaea CREB2 is homologous to transcriptional repressors, human CREB2, mouse activating transcription factor-4, and Aplysia CREB2 in the bZIP domain. In situ hybridization revealed that only a relatively few neurons showed strongly positive signals for Lymnaea CREB1 mRNA, whereas all the neurons in the CNS contained Lymnaea CREB2 mRNA. Using one of the neurons (the cerebral giant cell) containing Lymnaea CREB1 mRNA, we showed that the injection of a CRE oligonucleotide inhibited a cAMP-induced, long-lasting synaptic plasticity. We therefore conclude that CREBs are present in Lymnaea neurons and may function as necessary players in behavioral plasticity.
| Original language | English |
|---|---|
| Pages (from-to) | 455-466 |
| Number of pages | 12 |
| Journal | Journal of Neurobiology |
| Volume | 58 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2004 Mar |
| Externally published | Yes |
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Keywords
- Associative learning
- Cerebral giant cell
- Cyclic AMP
- Facilitation
- Long-term memory
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
CREB in the Pond Snail Lymnaea stagnalis : Cloning, Gene Expression and Function in Identifiable Neurons of the Central Nervous System. / Sadamoto, Hisayo; Sato, Hanae; Kobayashi, Suguru; Murakami, Jun; Aonuma, Hitoshi; Ando, Hironori; Fujito, Yutaka; Hamano, Kaoru; Awaji, Masahiko; Lukowiak, Ken; Urano, Akihisa; Ito, Etsuro.
In: Journal of Neurobiology, Vol. 58, No. 4, 03.2004, p. 455-466.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CREB in the Pond Snail Lymnaea stagnalis
T2 - Cloning, Gene Expression and Function in Identifiable Neurons of the Central Nervous System
AU - Sadamoto, Hisayo
AU - Sato, Hanae
AU - Kobayashi, Suguru
AU - Murakami, Jun
AU - Aonuma, Hitoshi
AU - Ando, Hironori
AU - Fujito, Yutaka
AU - Hamano, Kaoru
AU - Awaji, Masahiko
AU - Lukowiak, Ken
AU - Urano, Akihisa
AU - Ito, Etsuro
PY - 2004/3
Y1 - 2004/3
N2 - The pond snail Lymnaea stagnalis is an excellent model system in which to study the neuronal and molecular substrates of associative learning and its consolidation into long-term memory. Until now, the presence of cyclic AMP (cAMP)-responsive element binding protein (CREB), which is believed to be a necessary component in the process of a learned behavior that is consolidated into long-term memory, has only been assumed in Lymnaea neurons. We therefore cloned and analyzed the cDNA sequences of homologues of CREB1 and CREB2 and determined the presence of these mRNAs in identifiable neurons of the central nervous system (CNS) of L. stagnalis. The deduced amino acid sequence of Lymnaea CREB1 is homologous to transcriptional activators, mammalian CREB1 and Aplysia CREB1a, in the C-terminal DNA binding (bZIP) and phosphorylation domains, whereas the deduced amino acid sequence of Lymnaea CREB2 is homologous to transcriptional repressors, human CREB2, mouse activating transcription factor-4, and Aplysia CREB2 in the bZIP domain. In situ hybridization revealed that only a relatively few neurons showed strongly positive signals for Lymnaea CREB1 mRNA, whereas all the neurons in the CNS contained Lymnaea CREB2 mRNA. Using one of the neurons (the cerebral giant cell) containing Lymnaea CREB1 mRNA, we showed that the injection of a CRE oligonucleotide inhibited a cAMP-induced, long-lasting synaptic plasticity. We therefore conclude that CREBs are present in Lymnaea neurons and may function as necessary players in behavioral plasticity.
AB - The pond snail Lymnaea stagnalis is an excellent model system in which to study the neuronal and molecular substrates of associative learning and its consolidation into long-term memory. Until now, the presence of cyclic AMP (cAMP)-responsive element binding protein (CREB), which is believed to be a necessary component in the process of a learned behavior that is consolidated into long-term memory, has only been assumed in Lymnaea neurons. We therefore cloned and analyzed the cDNA sequences of homologues of CREB1 and CREB2 and determined the presence of these mRNAs in identifiable neurons of the central nervous system (CNS) of L. stagnalis. The deduced amino acid sequence of Lymnaea CREB1 is homologous to transcriptional activators, mammalian CREB1 and Aplysia CREB1a, in the C-terminal DNA binding (bZIP) and phosphorylation domains, whereas the deduced amino acid sequence of Lymnaea CREB2 is homologous to transcriptional repressors, human CREB2, mouse activating transcription factor-4, and Aplysia CREB2 in the bZIP domain. In situ hybridization revealed that only a relatively few neurons showed strongly positive signals for Lymnaea CREB1 mRNA, whereas all the neurons in the CNS contained Lymnaea CREB2 mRNA. Using one of the neurons (the cerebral giant cell) containing Lymnaea CREB1 mRNA, we showed that the injection of a CRE oligonucleotide inhibited a cAMP-induced, long-lasting synaptic plasticity. We therefore conclude that CREBs are present in Lymnaea neurons and may function as necessary players in behavioral plasticity.
KW - Associative learning
KW - Cerebral giant cell
KW - Cyclic AMP
KW - Facilitation
KW - Long-term memory
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U2 - 10.1002/neu.10296
DO - 10.1002/neu.10296
M3 - Article
VL - 58
SP - 455
EP - 466
JO - Developmental Neurobiology
JF - Developmental Neurobiology
SN - 1932-8451
IS - 4
ER -