CRMP4 suppresses apical dendrite bifurcation of CA1 pyramidal neurons in the mouse hippocampus

Emi Niisato, Jun Nagai, Naoya Yamashita, Takaya Abe, Hiroshi Kiyonari, Yoshio Goshima, Toshio Ohshima

    Research output: Contribution to journalArticle

    39 Citations (Scopus)

    Abstract

    Collapsin response mediator proteins (CRMPs) are a family of cytosolic phosphoproteins that consist of 5 members (CRMP 1-5). CRMP2 and CRMP4 regulate neurite outgrowth by binding to tubulin heterodimers, resulting in the assembly of microtubules. CRMP2 also mediates the growth cone collapse response to the repulsive guidance molecule semaphorin-3A (Sema3A). However, the role of CRMP4 in Sema3A signaling and its function in the developing mouse brain remain unclear. We generated CRMP4-/- mice in order to study the in vivo function of CRMP4 and identified a phenotype of proximal bifurcation of apical dendrites in the CA1 pyramidal neurons of CRMP4-/- mice. We also observed increased dendritic branching in cultured CRMP4-/- hippocampal neurons as well as in cultured cortical neurons treated with CRMP4 shRNA. Sema3A induces extension and branching of the dendrites of hippocampal neurons; however, these inductions were compromised in the CRMP4-/- hippocampal neurons. These results suggest that CRMP4 suppresses apical dendrite bifurcation of CA1 pyramidal neurons in the mouse hippocampus and that this is partly dependent on Sema3A signaling.

    Original languageEnglish
    Pages (from-to)1447-1457
    Number of pages11
    JournalDevelopmental Neurobiology
    Volume72
    Issue number11
    DOIs
    Publication statusPublished - 2012 Nov

      Fingerprint

    Keywords

    • CRMP
    • Dendrite
    • Hippocampus
    • Mouse brain development
    • Sema3A

    ASJC Scopus subject areas

    • Cellular and Molecular Neuroscience
    • Developmental Neuroscience

    Cite this