Crystal structure analysis of the translation factor RF3 (release factor 3)

Kiyohito Kihira, Yoshihiro Shimizu, Yasuhito Shomura, Naoki Shibata, Masaya Kitamura, Atsushi Nakagawa, Takuya Ueda, Kozo Ochi, Yoshiki Higuchi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The bacterial translational GTPases release factor RF3 promotes translation termination by recycling RF1 or RF2. Here, we present the crystal structures of RF3 complexed with GDP and guanosine 3′,5′-(bis)diphosphate (ppGpp) at resolutions of 1.8 and 3.0 Å, respectively. ppGpp is involved in the so-called "stringent response" of bacteria. ppGpp binds at the same site as GDP, suggesting that GDP and ppGpp are two alternative physiologically relevant ligands of RF3. We also found that ppGpp decelerates the recycling of RF1 by RF3. These lines of evidence suggest that RF3 functions both as a cellular metabolic sensor and as a regulator.

Original languageEnglish
Pages (from-to)3705-3709
Number of pages5
JournalFEBS Letters
Volume586
Issue number20
DOIs
Publication statusPublished - 2012 Oct 19
Externally publishedYes

Fingerprint

Statistical Factor Analysis
Crystal structure
Recycling
Guanosine
Diphosphates
GTP Phosphohydrolases
Bacteria
peptide-chain-release factor 3
Ligands
Sensors

Keywords

  • GTPase
  • Release factor
  • Ribosome
  • Stringent response
  • Translation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Kihira, K., Shimizu, Y., Shomura, Y., Shibata, N., Kitamura, M., Nakagawa, A., ... Higuchi, Y. (2012). Crystal structure analysis of the translation factor RF3 (release factor 3). FEBS Letters, 586(20), 3705-3709. https://doi.org/10.1016/j.febslet.2012.08.029

Crystal structure analysis of the translation factor RF3 (release factor 3). / Kihira, Kiyohito; Shimizu, Yoshihiro; Shomura, Yasuhito; Shibata, Naoki; Kitamura, Masaya; Nakagawa, Atsushi; Ueda, Takuya; Ochi, Kozo; Higuchi, Yoshiki.

In: FEBS Letters, Vol. 586, No. 20, 19.10.2012, p. 3705-3709.

Research output: Contribution to journalArticle

Kihira, K, Shimizu, Y, Shomura, Y, Shibata, N, Kitamura, M, Nakagawa, A, Ueda, T, Ochi, K & Higuchi, Y 2012, 'Crystal structure analysis of the translation factor RF3 (release factor 3)', FEBS Letters, vol. 586, no. 20, pp. 3705-3709. https://doi.org/10.1016/j.febslet.2012.08.029
Kihira K, Shimizu Y, Shomura Y, Shibata N, Kitamura M, Nakagawa A et al. Crystal structure analysis of the translation factor RF3 (release factor 3). FEBS Letters. 2012 Oct 19;586(20):3705-3709. https://doi.org/10.1016/j.febslet.2012.08.029
Kihira, Kiyohito ; Shimizu, Yoshihiro ; Shomura, Yasuhito ; Shibata, Naoki ; Kitamura, Masaya ; Nakagawa, Atsushi ; Ueda, Takuya ; Ochi, Kozo ; Higuchi, Yoshiki. / Crystal structure analysis of the translation factor RF3 (release factor 3). In: FEBS Letters. 2012 ; Vol. 586, No. 20. pp. 3705-3709.
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