Crystal structure of the nucleosome containing histone H3 with crotonylated lysine 122

Yuya Suzuki, Naoki Horikoshi, Daiki Kato, Hitoshi Kurumizaka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The crotonylation of histones is an important post-translational modification, and epigenetically functions in the regulation of genomic DNA activity. The histone modifications in the structured "histone-fold" domains are considered to have an especially important impact on the nucleosome structure and dynamics. In the present study, we reconstituted the human nucleosome containing histone H3.2 crotonylated at the Lys122 residue, and determined its crystal structure at 2.56 Å resolution. We found that the crotonylation of the H3 Lys122 residue does not affect the overall nucleosome structure, but locally impedes the formation of the water-mediated hydrogen bond with the DNA backbone. Consistently, thermal stability assays revealed that the H3 Lys122 crotonylation, as well as the H3 Lys122 acetylation, clearly reduced the histone-DNA association.

Original languageEnglish
Pages (from-to)483-489
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume469
Issue number3
DOIs
Publication statusPublished - 2016 Jan 15

Fingerprint

Nucleosomes
Histones
Lysine
Crystal structure
DNA
Histone Code
Post Translational Protein Processing
Acetylation
Hydrogen
Hot Temperature
Assays
Hydrogen bonds
Thermodynamic stability
Water
Association reactions

Keywords

  • Acetylation
  • Chromatin
  • Crotonylation
  • Crystal structure
  • Histone post-translational modification
  • Nucleosome

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Crystal structure of the nucleosome containing histone H3 with crotonylated lysine 122. / Suzuki, Yuya; Horikoshi, Naoki; Kato, Daiki; Kurumizaka, Hitoshi.

In: Biochemical and Biophysical Research Communications, Vol. 469, No. 3, 15.01.2016, p. 483-489.

Research output: Contribution to journalArticle

Suzuki, Yuya ; Horikoshi, Naoki ; Kato, Daiki ; Kurumizaka, Hitoshi. / Crystal structure of the nucleosome containing histone H3 with crotonylated lysine 122. In: Biochemical and Biophysical Research Communications. 2016 ; Vol. 469, No. 3. pp. 483-489.
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