Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB8

Ami Matsumoto, Yoshihiro Shimizu, Chie Takemoto, Takuya Ueda, Toshio Uchiumi, Kosuke Ito

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Peptidyl-tRNA is produced from the ribosome as a result of aborted translation. Peptidyl-tRNA hydrolase cleaves the ester bond between the peptide and the tRNA of peptidyl-tRNA molecules, to recycle tRNA for further rounds of protein synthesis. In this study, peptidyl-tRNA hydrolase from Thermus thermophilus HB8 (TthPth) was crystallized using 2-methyl-2,4-pentanediol as a precipitant. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 47.45, b = 53.92, c = 58.67Å, and diffracted X-rays to atomic resolution (beyond 1.0Å resolution). The asymmetric unit is expected to contain one TthPth molecule, with a solvent content of 27.13% (V M = 1.69Å3Da-1). The structure is being solved by molecular replacement.

Original languageEnglish
Pages (from-to)332-335
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume69
Issue number3
DOIs
Publication statusPublished - 2013 Mar 1
Externally publishedYes

Fingerprint

Thermus thermophilus
X ray analysis
Transfer RNA
Crystallization
X-Rays
crystallization
protein synthesis
ribosomes
Molecules
Ribosomes
peptides
esters
molecules
Esters
x rays
X rays
Peptides
Crystals
cells
crystals

Keywords

  • peptidyl-tRNA hydrolase
  • Thermus thermophilus

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB8. / Matsumoto, Ami; Shimizu, Yoshihiro; Takemoto, Chie; Ueda, Takuya; Uchiumi, Toshio; Ito, Kosuke.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 69, No. 3, 01.03.2013, p. 332-335.

Research output: Contribution to journalArticle

Matsumoto, Ami ; Shimizu, Yoshihiro ; Takemoto, Chie ; Ueda, Takuya ; Uchiumi, Toshio ; Ito, Kosuke. / Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB8. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2013 ; Vol. 69, No. 3. pp. 332-335.
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