Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB8

Ami Matsumoto; Yoshihiro Shimizu; Chie Takemoto; Takuya Ueda; Toshio Uchiumi; Kosuke Ito

doi:10.1107/S1744309113003424

Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB8

Ami Matsumoto, Yoshihiro Shimizu, Chie Takemoto, Takuya Ueda, Toshio Uchiumi, Kosuke Ito

Research output: Contribution to journal › Article

1 Citation (Scopus)

Abstract

Peptidyl-tRNA is produced from the ribosome as a result of aborted translation. Peptidyl-tRNA hydrolase cleaves the ester bond between the peptide and the tRNA of peptidyl-tRNA molecules, to recycle tRNA for further rounds of protein synthesis. In this study, peptidyl-tRNA hydrolase from Thermus thermophilus HB8 (TthPth) was crystallized using 2-methyl-2,4-pentanediol as a precipitant. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 47.45, b = 53.92, c = 58.67Å, and diffracted X-rays to atomic resolution (beyond 1.0Å resolution). The asymmetric unit is expected to contain one TthPth molecule, with a solvent content of 27.13% (V M = 1.69Å³Da^-1). The structure is being solved by molecular replacement.

Original language	English
Pages (from-to)	332-335
Number of pages	4
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	69
Issue number	3
DOIs	https://doi.org/10.1107/S1744309113003424
Publication status	Published - 2013 Mar 1
Externally published	Yes

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Keywords

peptidyl-tRNA hydrolase
Thermus thermophilus

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics
Condensed Matter Physics

Cite this

Matsumoto, A., Shimizu, Y., Takemoto, C., Ueda, T., Uchiumi, T., & Ito, K. (2013). Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB8. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 69(3), 332-335. https://doi.org/10.1107/S1744309113003424

Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB8. / Matsumoto, Ami; Shimizu, Yoshihiro; Takemoto, Chie; Ueda, Takuya; Uchiumi, Toshio; Ito, Kosuke.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 69, No. 3, 01.03.2013, p. 332-335.

Research output: Contribution to journal › Article

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10.1107/S1744309113003424