Cullin-3/KCTD10 E3 complex is essential for Rac1 activation through RhoB degradation in human epidermal growth factor receptor 2-positive breast cancer cells

Akari Murakami, Masashi Maekawa, Katsuhisa Kawai, Jun Nakayama, Nobukazu Araki, Kentaro Senba, Tomohiko Taguchi, Yoshiaki Kamei, Yasutsugu Takada, Shigeki Higashiyama

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Rho GTPase Rac1 is a central regulator of F-actin organization and signal transduction to control plasma membrane dynamics and cell proliferation. Dysregulated Rac1 activity is often observed in various cancers including breast cancer and is suggested to be critical for malignancy. Here, we showed that the ubiquitin E3 ligase complex Cullin-3 (CUL3)/KCTD10 is essential for epidermal growth factor (EGF)-induced/human epidermal growth factor receptor 2 (HER2)-dependent Rac1 activation in HER2-positive breast cancer cells. EGF-induced dorsal membrane ruffle formation and cell proliferation that depends on both Rac1 and HER2 were suppressed in CUL3- or KCTD10-depleted cells. Mechanistically, CUL3/KCTD10 ubiquitinated RhoB for degradation, another Rho GTPase that inhibits Rac1 activation at the plasma membrane by suppressing endosome-to-plasma membrane traffic of Rac1. In HER2-positive breast cancers, high expression of Rac1 mRNA significantly correlated with poor prognosis of the patients. This study shows that this novel molecular axis (CUL3/KCTD10/RhoB) positively regulates the activity of Rac1 in HER2-positive breast cancers, and our findings may lead to new treatment options for HER2- and Rac1-positive breast cancers.

    Original languageEnglish
    Pages (from-to)650-661
    Number of pages12
    JournalCancer Science
    Volume110
    Issue number2
    DOIs
    Publication statusPublished - 2019 Feb 1

    Fingerprint

    Cullin Proteins
    Breast Neoplasms
    rho GTP-Binding Proteins
    Cell Membrane
    Epidermal Growth Factor
    Cell Proliferation
    Ubiquitin-Protein Ligases
    Endosomes
    Plasma Cells
    human ERBB2 protein
    Actins
    Signal Transduction
    Messenger RNA
    Membranes

    Keywords

    • Cullin-3
    • HER2
    • membrane ruffle
    • Rac1
    • RhoB

    ASJC Scopus subject areas

    • Oncology
    • Cancer Research

    Cite this

    Cullin-3/KCTD10 E3 complex is essential for Rac1 activation through RhoB degradation in human epidermal growth factor receptor 2-positive breast cancer cells. / Murakami, Akari; Maekawa, Masashi; Kawai, Katsuhisa; Nakayama, Jun; Araki, Nobukazu; Senba, Kentaro; Taguchi, Tomohiko; Kamei, Yoshiaki; Takada, Yasutsugu; Higashiyama, Shigeki.

    In: Cancer Science, Vol. 110, No. 2, 01.02.2019, p. 650-661.

    Research output: Contribution to journalArticle

    Murakami, A, Maekawa, M, Kawai, K, Nakayama, J, Araki, N, Senba, K, Taguchi, T, Kamei, Y, Takada, Y & Higashiyama, S 2019, 'Cullin-3/KCTD10 E3 complex is essential for Rac1 activation through RhoB degradation in human epidermal growth factor receptor 2-positive breast cancer cells' Cancer Science, vol. 110, no. 2, pp. 650-661. https://doi.org/10.1111/cas.13899
    Murakami, Akari ; Maekawa, Masashi ; Kawai, Katsuhisa ; Nakayama, Jun ; Araki, Nobukazu ; Senba, Kentaro ; Taguchi, Tomohiko ; Kamei, Yoshiaki ; Takada, Yasutsugu ; Higashiyama, Shigeki. / Cullin-3/KCTD10 E3 complex is essential for Rac1 activation through RhoB degradation in human epidermal growth factor receptor 2-positive breast cancer cells. In: Cancer Science. 2019 ; Vol. 110, No. 2. pp. 650-661.
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