Culture-independent method for screening and identifying microbial enzyme-encoding genes using microdroplet-based single cell genomics

K. Nakamura, R. Iizuka, T. Yoshida, Y. Hatada, Y. Takaki, S. Nishi, A. Iguchi, Donghyun Yoon, Tetsushi Sekiguchi, Shuichi Shoji, T. Funatsu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Environmental microbes are a great source of industrially valuable enzymes with high and unique catalytic activities. However, a vast majority of microbes remain unculturable and thus are not accessible by culture-based methods. Here, we present a rapid and efficient method to screen and identify enzyme-encoding genes from environmental microbes in a culture-independent manner. This method combines activity-based single cell screening using microdroplets and single cell genomics. Using this method, we successfully identified 13 novel β-glucosidase genes from uncultured marine bacteria. This method will facilitate the identification of genes encoding industrially valuable enzymes.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages513-515
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

Fingerprint

Gene encoding
Screening
Enzymes
Catalyst activity
Bacteria
Genes
Genomics

Keywords

  • Enzyme
  • Microdroplet
  • Screening
  • Single cell genomics

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Nakamura, K., Iizuka, R., Yoshida, T., Hatada, Y., Takaki, Y., Nishi, S., ... Funatsu, T. (2015). Culture-independent method for screening and identifying microbial enzyme-encoding genes using microdroplet-based single cell genomics. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 513-515). Chemical and Biological Microsystems Society.

Culture-independent method for screening and identifying microbial enzyme-encoding genes using microdroplet-based single cell genomics. / Nakamura, K.; Iizuka, R.; Yoshida, T.; Hatada, Y.; Takaki, Y.; Nishi, S.; Iguchi, A.; Yoon, Donghyun; Sekiguchi, Tetsushi; Shoji, Shuichi; Funatsu, T.

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 513-515.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nakamura, K, Iizuka, R, Yoshida, T, Hatada, Y, Takaki, Y, Nishi, S, Iguchi, A, Yoon, D, Sekiguchi, T, Shoji, S & Funatsu, T 2015, Culture-independent method for screening and identifying microbial enzyme-encoding genes using microdroplet-based single cell genomics. in MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 513-515, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 15/10/25.
Nakamura K, Iizuka R, Yoshida T, Hatada Y, Takaki Y, Nishi S et al. Culture-independent method for screening and identifying microbial enzyme-encoding genes using microdroplet-based single cell genomics. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2015. p. 513-515
Nakamura, K. ; Iizuka, R. ; Yoshida, T. ; Hatada, Y. ; Takaki, Y. ; Nishi, S. ; Iguchi, A. ; Yoon, Donghyun ; Sekiguchi, Tetsushi ; Shoji, Shuichi ; Funatsu, T. / Culture-independent method for screening and identifying microbial enzyme-encoding genes using microdroplet-based single cell genomics. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. pp. 513-515
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