Cyclic Peptides for Efficient Detection of Collagen

Koh K. Takita, Kazunori K. Fujii, Tetsuya Kadonosono, Ryo Masuda, Takaki Koide

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We report here a new class of collagen-binding peptides, cyclic collagen-mimetic peptides (cCMPs), that efficiently hybridize with the triple-helix-forming portions of collagen. cCMPs are composed of two parallel collagen-like (Xaa-Yaa-Gly)n strands with both termini tethered by covalent linkages. Enzyme-linked immunosorbent assays and western blotting analysis showed that cCMPs exhibit more potent affinity toward collagen than reported collagen-binding peptides and can specifically detect different collagen polypeptides in a mixture of proteins. Collagen secreted from cultured cells was detected by confocal microscopy with fluorescein-labeled cCMP. The cCMP is also shown to detect sensitively folding intermediates in the endoplasmic reticulum, something that was difficult to visualize with conventional collagen detectors. Molecular-dynamics simulations suggested that a cCMP forms a more stably hybridized product than its single-chain counterpart; this could explain why cCMP has higher affinity toward denatured collagen. These results indicate the usefulness of cCMPs as tools for detecting denatured collagen.

Original languageEnglish
Pages (from-to)1613-1617
Number of pages5
JournalChemBioChem
Volume19
Issue number15
DOIs
Publication statusPublished - 2018 Aug 6

Keywords

  • collagen
  • collagen-mimetic peptide
  • hybridization
  • triple helixes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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