Cytokine responses to carbohydrate ingestion during recovery from exercise-induced muscle injury

Megan L R Ross, Shona L. Halson, Katsuhiko Suzuki, Andrew Garnham, John A. Hawley, David Cameron-Smith, Jonathan M. Peake

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We investigated the effect of carbohydrate ingestion after maximal lengthening contractions of the knee extensors on circulating concentrations of myocellular proteins and cytokines, and cytokine mRNA expression in muscle. Using a cross-over design, 10 healthy males completed 5 sets of 10 lengthening (eccentric) contractions (unilateral leg press) at 120% 1 repetition-maximum. Subjects were randomized to consume a carbohydrate drink (15% weight per volume; 3 g/kg BM) for 3 h after exercise using one leg, or a placebo drink after exercise using the contralateral leg on another day. Blood samples (10 mL) were collected before exercise and after 0, 30, 60, 90, 120, 150, and 180 min of recovery. Muscle biopsies (vastus lateralis) were collected before exercise and after 3 h of recovery. Following carbohydrate ingestion, serum concentrations of glucose (30-90 min and at 150 min) and insulin (30-180 min) increased (P <0.05) above pre-exercise values. Serum myoglobin concentration increased (∼250%; P<0.05) after both trials. In contrast, serum cytokine concentrations were unchanged throughout recovery in both trials. Muscle mRNA expression for IL-8 (6.4-fold), MCP-1 (4.7-fold), and IL-6 (7.3-fold) increased substantially after carbohydrate ingestion. TNF-α mRNA expression did not change after either trial. Carbohydrate ingestion during early recovery from exercise-induced muscle injury may promote proinflammatory reactions within skeletal muscle.

Original languageEnglish
Pages (from-to)329-337
Number of pages9
JournalJournal of Interferon and Cytokine Research
Volume30
Issue number5
DOIs
Publication statusPublished - 2010 May 1

Fingerprint

Eating
Carbohydrates
Cytokines
Muscles
Wounds and Injuries
Leg
Messenger RNA
Serum
Myoglobin
Quadriceps Muscle
Interleukin-8
Cross-Over Studies
Interleukin-6
Knee
Skeletal Muscle
Placebos
Insulin
Biopsy
Weights and Measures
Glucose

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Cell Biology

Cite this

Cytokine responses to carbohydrate ingestion during recovery from exercise-induced muscle injury. / Ross, Megan L R; Halson, Shona L.; Suzuki, Katsuhiko; Garnham, Andrew; Hawley, John A.; Cameron-Smith, David; Peake, Jonathan M.

In: Journal of Interferon and Cytokine Research, Vol. 30, No. 5, 01.05.2010, p. 329-337.

Research output: Contribution to journalArticle

Ross, Megan L R ; Halson, Shona L. ; Suzuki, Katsuhiko ; Garnham, Andrew ; Hawley, John A. ; Cameron-Smith, David ; Peake, Jonathan M. / Cytokine responses to carbohydrate ingestion during recovery from exercise-induced muscle injury. In: Journal of Interferon and Cytokine Research. 2010 ; Vol. 30, No. 5. pp. 329-337.
@article{529d75029f7f4c3aac20fe7aa60d5f41,
title = "Cytokine responses to carbohydrate ingestion during recovery from exercise-induced muscle injury",
abstract = "We investigated the effect of carbohydrate ingestion after maximal lengthening contractions of the knee extensors on circulating concentrations of myocellular proteins and cytokines, and cytokine mRNA expression in muscle. Using a cross-over design, 10 healthy males completed 5 sets of 10 lengthening (eccentric) contractions (unilateral leg press) at 120{\%} 1 repetition-maximum. Subjects were randomized to consume a carbohydrate drink (15{\%} weight per volume; 3 g/kg BM) for 3 h after exercise using one leg, or a placebo drink after exercise using the contralateral leg on another day. Blood samples (10 mL) were collected before exercise and after 0, 30, 60, 90, 120, 150, and 180 min of recovery. Muscle biopsies (vastus lateralis) were collected before exercise and after 3 h of recovery. Following carbohydrate ingestion, serum concentrations of glucose (30-90 min and at 150 min) and insulin (30-180 min) increased (P <0.05) above pre-exercise values. Serum myoglobin concentration increased (∼250{\%}; P<0.05) after both trials. In contrast, serum cytokine concentrations were unchanged throughout recovery in both trials. Muscle mRNA expression for IL-8 (6.4-fold), MCP-1 (4.7-fold), and IL-6 (7.3-fold) increased substantially after carbohydrate ingestion. TNF-α mRNA expression did not change after either trial. Carbohydrate ingestion during early recovery from exercise-induced muscle injury may promote proinflammatory reactions within skeletal muscle.",
author = "Ross, {Megan L R} and Halson, {Shona L.} and Katsuhiko Suzuki and Andrew Garnham and Hawley, {John A.} and David Cameron-Smith and Peake, {Jonathan M.}",
year = "2010",
month = "5",
day = "1",
doi = "10.1089/jir.2009.0079",
language = "English",
volume = "30",
pages = "329--337",
journal = "Journal of Interferon and Cytokine Research",
issn = "1079-9907",
publisher = "Mary Ann Liebert Inc.",
number = "5",

}

TY - JOUR

T1 - Cytokine responses to carbohydrate ingestion during recovery from exercise-induced muscle injury

AU - Ross, Megan L R

AU - Halson, Shona L.

AU - Suzuki, Katsuhiko

AU - Garnham, Andrew

AU - Hawley, John A.

AU - Cameron-Smith, David

AU - Peake, Jonathan M.

PY - 2010/5/1

Y1 - 2010/5/1

N2 - We investigated the effect of carbohydrate ingestion after maximal lengthening contractions of the knee extensors on circulating concentrations of myocellular proteins and cytokines, and cytokine mRNA expression in muscle. Using a cross-over design, 10 healthy males completed 5 sets of 10 lengthening (eccentric) contractions (unilateral leg press) at 120% 1 repetition-maximum. Subjects were randomized to consume a carbohydrate drink (15% weight per volume; 3 g/kg BM) for 3 h after exercise using one leg, or a placebo drink after exercise using the contralateral leg on another day. Blood samples (10 mL) were collected before exercise and after 0, 30, 60, 90, 120, 150, and 180 min of recovery. Muscle biopsies (vastus lateralis) were collected before exercise and after 3 h of recovery. Following carbohydrate ingestion, serum concentrations of glucose (30-90 min and at 150 min) and insulin (30-180 min) increased (P <0.05) above pre-exercise values. Serum myoglobin concentration increased (∼250%; P<0.05) after both trials. In contrast, serum cytokine concentrations were unchanged throughout recovery in both trials. Muscle mRNA expression for IL-8 (6.4-fold), MCP-1 (4.7-fold), and IL-6 (7.3-fold) increased substantially after carbohydrate ingestion. TNF-α mRNA expression did not change after either trial. Carbohydrate ingestion during early recovery from exercise-induced muscle injury may promote proinflammatory reactions within skeletal muscle.

AB - We investigated the effect of carbohydrate ingestion after maximal lengthening contractions of the knee extensors on circulating concentrations of myocellular proteins and cytokines, and cytokine mRNA expression in muscle. Using a cross-over design, 10 healthy males completed 5 sets of 10 lengthening (eccentric) contractions (unilateral leg press) at 120% 1 repetition-maximum. Subjects were randomized to consume a carbohydrate drink (15% weight per volume; 3 g/kg BM) for 3 h after exercise using one leg, or a placebo drink after exercise using the contralateral leg on another day. Blood samples (10 mL) were collected before exercise and after 0, 30, 60, 90, 120, 150, and 180 min of recovery. Muscle biopsies (vastus lateralis) were collected before exercise and after 3 h of recovery. Following carbohydrate ingestion, serum concentrations of glucose (30-90 min and at 150 min) and insulin (30-180 min) increased (P <0.05) above pre-exercise values. Serum myoglobin concentration increased (∼250%; P<0.05) after both trials. In contrast, serum cytokine concentrations were unchanged throughout recovery in both trials. Muscle mRNA expression for IL-8 (6.4-fold), MCP-1 (4.7-fold), and IL-6 (7.3-fold) increased substantially after carbohydrate ingestion. TNF-α mRNA expression did not change after either trial. Carbohydrate ingestion during early recovery from exercise-induced muscle injury may promote proinflammatory reactions within skeletal muscle.

UR - http://www.scopus.com/inward/record.url?scp=77952577687&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952577687&partnerID=8YFLogxK

U2 - 10.1089/jir.2009.0079

DO - 10.1089/jir.2009.0079

M3 - Article

C2 - 20187772

AN - SCOPUS:77952577687

VL - 30

SP - 329

EP - 337

JO - Journal of Interferon and Cytokine Research

JF - Journal of Interferon and Cytokine Research

SN - 1079-9907

IS - 5

ER -