Cytokine responses to carbohydrate ingestion during recovery from exercise-induced muscle injury

Megan L.R. Ross, Shona L. Halson, Katsuhiko Suzuki, Andrew Garnham, John A. Hawley, David Cameron-Smith, Jonathan M. Peake

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We investigated the effect of carbohydrate ingestion after maximal lengthening contractions of the knee extensors on circulating concentrations of myocellular proteins and cytokines, and cytokine mRNA expression in muscle. Using a cross-over design, 10 healthy males completed 5 sets of 10 lengthening (eccentric) contractions (unilateral leg press) at 120% 1 repetition-maximum. Subjects were randomized to consume a carbohydrate drink (15% weight per volume; 3 g/kg BM) for 3 h after exercise using one leg, or a placebo drink after exercise using the contralateral leg on another day. Blood samples (10 mL) were collected before exercise and after 0, 30, 60, 90, 120, 150, and 180 min of recovery. Muscle biopsies (vastus lateralis) were collected before exercise and after 3 h of recovery. Following carbohydrate ingestion, serum concentrations of glucose (30-90 min and at 150 min) and insulin (30-180 min) increased (P <0.05) above pre-exercise values. Serum myoglobin concentration increased (∼250%; P<0.05) after both trials. In contrast, serum cytokine concentrations were unchanged throughout recovery in both trials. Muscle mRNA expression for IL-8 (6.4-fold), MCP-1 (4.7-fold), and IL-6 (7.3-fold) increased substantially after carbohydrate ingestion. TNF-α mRNA expression did not change after either trial. Carbohydrate ingestion during early recovery from exercise-induced muscle injury may promote proinflammatory reactions within skeletal muscle.

Original languageEnglish
Pages (from-to)329-337
Number of pages9
JournalJournal of Interferon and Cytokine Research
Volume30
Issue number5
DOIs
Publication statusPublished - 2010 May 1

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Virology

Fingerprint Dive into the research topics of 'Cytokine responses to carbohydrate ingestion during recovery from exercise-induced muscle injury'. Together they form a unique fingerprint.

  • Cite this