Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1

Takeyuki Suzuki, Yoshiko Okamura, Atsushi Arakaki, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A non-magnetic mutant of Magnetospirillum magneticum AMB-1 (NMA61), harboring a defective gene located in ORF4 (gene ID: amb4111) was generated by transposon mutagenesis. Biochemical characterization of the gene product of ORF4 revealed that it was localized in the cytoplasm and displayed ATPase activity. The ability of NMA61 to take up iron was severely compromised. Ferrous ion concentration in the medium decreased more with the wild-type than with NMA61, while the iron content in the cytoplasmic fraction of NMA61 was much lower than the wild-type strain. This cytoplasmic ATPase is essential for iron trafficking within M. magneticum AMB-1.

Original languageEnglish
Pages (from-to)3443-3448
Number of pages6
JournalFEBS Letters
Volume581
Issue number18
DOIs
Publication statusPublished - 2007 Jul 24
Externally publishedYes

Fingerprint

Magnetospirillum
Adenosine Triphosphatases
Bacteria
Iron
Genes
Ions
Mutagenesis
Cytoplasm

Keywords

  • ATPase
  • Cytoplasmic iron concentration
  • Ferrous ion transport
  • Magnetospirillum magneticum AMB-1
  • Magnetotactic bacteria

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1. / Suzuki, Takeyuki; Okamura, Yoshiko; Arakaki, Atsushi; Takeyama, Haruko; Matsunaga, Tadashi.

In: FEBS Letters, Vol. 581, No. 18, 24.07.2007, p. 3443-3448.

Research output: Contribution to journalArticle

Suzuki, Takeyuki ; Okamura, Yoshiko ; Arakaki, Atsushi ; Takeyama, Haruko ; Matsunaga, Tadashi. / Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1. In: FEBS Letters. 2007 ; Vol. 581, No. 18. pp. 3443-3448.
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