Cytoplasmic Streaming Velocity as a Plant Size Determinant

Motoki Tominaga, Atsushi Kimura, Etsuo Yokota, Takeshi Haraguchi, Teruo Shimmen, Keiichi Yamamoto, Akihiko Nakano, Kohji Ito

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Cytoplasmic streaming is active transport widely occurring in plant cells ranging from algae to angiosperms. Although it has been revealed that cytoplasmic streaming is generated by organelle-associated myosin XI moving along actin bundles, the fundamental function in plants remains unclear. We generated high- and low-speed chimeric myosin XI by replacing the motor domains of Arabidopsis thaliana myosin XI-2 with those of Chara corallina myosin XI and Homo sapiens myosin Vb, respectively. Surprisingly, the plant sizes of the transgenic. Arabidopsis expressing high- and low-speed chimeric myosin XI-2 were larger and smaller, respectively, than that of the wild-type plant. This size change correlated with acceleration and deceleration, respectively, of cytoplasmic streaming. Our results strongly suggest that cytoplasmic streaming is a key determinant of plant size. Furthermore, because cytoplasmic streaming is a common system for intracellular transport in plants, our system could have applications in artificial size control in plants.

Original languageEnglish
Pages (from-to)345-352
Number of pages8
JournalDevelopmental Cell
Volume27
Issue number3
DOIs
Publication statusPublished - 2013 Nov 11
Externally publishedYes

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Cytoplasmic Streaming
Myosins
Arabidopsis
Chara
Angiosperms
Deceleration
Active Biological Transport
Genetically Modified Plants
Plant Cells
Organelles
Algae
Actins

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Tominaga, M., Kimura, A., Yokota, E., Haraguchi, T., Shimmen, T., Yamamoto, K., ... Ito, K. (2013). Cytoplasmic Streaming Velocity as a Plant Size Determinant. Developmental Cell, 27(3), 345-352. https://doi.org/10.1016/j.devcel.2013.10.005

Cytoplasmic Streaming Velocity as a Plant Size Determinant. / Tominaga, Motoki; Kimura, Atsushi; Yokota, Etsuo; Haraguchi, Takeshi; Shimmen, Teruo; Yamamoto, Keiichi; Nakano, Akihiko; Ito, Kohji.

In: Developmental Cell, Vol. 27, No. 3, 11.11.2013, p. 345-352.

Research output: Contribution to journalArticle

Tominaga, M, Kimura, A, Yokota, E, Haraguchi, T, Shimmen, T, Yamamoto, K, Nakano, A & Ito, K 2013, 'Cytoplasmic Streaming Velocity as a Plant Size Determinant', Developmental Cell, vol. 27, no. 3, pp. 345-352. https://doi.org/10.1016/j.devcel.2013.10.005
Tominaga M, Kimura A, Yokota E, Haraguchi T, Shimmen T, Yamamoto K et al. Cytoplasmic Streaming Velocity as a Plant Size Determinant. Developmental Cell. 2013 Nov 11;27(3):345-352. https://doi.org/10.1016/j.devcel.2013.10.005
Tominaga, Motoki ; Kimura, Atsushi ; Yokota, Etsuo ; Haraguchi, Takeshi ; Shimmen, Teruo ; Yamamoto, Keiichi ; Nakano, Akihiko ; Ito, Kohji. / Cytoplasmic Streaming Velocity as a Plant Size Determinant. In: Developmental Cell. 2013 ; Vol. 27, No. 3. pp. 345-352.
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