Abstract
Cytoplasmic streaming is active transport widely occurring in plant cells ranging from algae to angiosperms. Although it has been revealed that cytoplasmic streaming is generated by organelle-associated myosin XI moving along actin bundles, the fundamental function in plants remains unclear. We generated high- and low-speed chimeric myosin XI by replacing the motor domains of Arabidopsis thaliana myosin XI-2 with those of Chara corallina myosin XI and Homo sapiens myosin Vb, respectively. Surprisingly, the plant sizes of the transgenic. Arabidopsis expressing high- and low-speed chimeric myosin XI-2 were larger and smaller, respectively, than that of the wild-type plant. This size change correlated with acceleration and deceleration, respectively, of cytoplasmic streaming. Our results strongly suggest that cytoplasmic streaming is a key determinant of plant size. Furthermore, because cytoplasmic streaming is a common system for intracellular transport in plants, our system could have applications in artificial size control in plants.
| Original language | English |
|---|---|
| Pages (from-to) | 345-352 |
| Number of pages | 8 |
| Journal | Developmental Cell |
| Volume | 27 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2013 Nov 11 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Developmental Biology
Cite this
Cytoplasmic Streaming Velocity as a Plant Size Determinant. / Tominaga, Motoki; Kimura, Atsushi; Yokota, Etsuo; Haraguchi, Takeshi; Shimmen, Teruo; Yamamoto, Keiichi; Nakano, Akihiko; Ito, Kohji.
In: Developmental Cell, Vol. 27, No. 3, 11.11.2013, p. 345-352.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cytoplasmic Streaming Velocity as a Plant Size Determinant
AU - Tominaga, Motoki
AU - Kimura, Atsushi
AU - Yokota, Etsuo
AU - Haraguchi, Takeshi
AU - Shimmen, Teruo
AU - Yamamoto, Keiichi
AU - Nakano, Akihiko
AU - Ito, Kohji
PY - 2013/11/11
Y1 - 2013/11/11
N2 - Cytoplasmic streaming is active transport widely occurring in plant cells ranging from algae to angiosperms. Although it has been revealed that cytoplasmic streaming is generated by organelle-associated myosin XI moving along actin bundles, the fundamental function in plants remains unclear. We generated high- and low-speed chimeric myosin XI by replacing the motor domains of Arabidopsis thaliana myosin XI-2 with those of Chara corallina myosin XI and Homo sapiens myosin Vb, respectively. Surprisingly, the plant sizes of the transgenic. Arabidopsis expressing high- and low-speed chimeric myosin XI-2 were larger and smaller, respectively, than that of the wild-type plant. This size change correlated with acceleration and deceleration, respectively, of cytoplasmic streaming. Our results strongly suggest that cytoplasmic streaming is a key determinant of plant size. Furthermore, because cytoplasmic streaming is a common system for intracellular transport in plants, our system could have applications in artificial size control in plants.
AB - Cytoplasmic streaming is active transport widely occurring in plant cells ranging from algae to angiosperms. Although it has been revealed that cytoplasmic streaming is generated by organelle-associated myosin XI moving along actin bundles, the fundamental function in plants remains unclear. We generated high- and low-speed chimeric myosin XI by replacing the motor domains of Arabidopsis thaliana myosin XI-2 with those of Chara corallina myosin XI and Homo sapiens myosin Vb, respectively. Surprisingly, the plant sizes of the transgenic. Arabidopsis expressing high- and low-speed chimeric myosin XI-2 were larger and smaller, respectively, than that of the wild-type plant. This size change correlated with acceleration and deceleration, respectively, of cytoplasmic streaming. Our results strongly suggest that cytoplasmic streaming is a key determinant of plant size. Furthermore, because cytoplasmic streaming is a common system for intracellular transport in plants, our system could have applications in artificial size control in plants.
UR - http://www.scopus.com/inward/record.url?scp=84887539785&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84887539785&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2013.10.005
DO - 10.1016/j.devcel.2013.10.005
M3 - Article
C2 - 24229646
AN - SCOPUS:84887539785
VL - 27
SP - 345
EP - 352
JO - Developmental Cell
JF - Developmental Cell
SN - 1534-5807
IS - 3
ER -