TY - JOUR
T1 - Cytoplasmic Streaming Velocity as a Plant Size Determinant
AU - Tominaga, Motoki
AU - Kimura, Atsushi
AU - Yokota, Etsuo
AU - Haraguchi, Takeshi
AU - Shimmen, Teruo
AU - Yamamoto, Keiichi
AU - Nakano, Akihiko
AU - Ito, Kohji
N1 - Funding Information:
We thank Dr. V.V. Dolja of Oregon State University for providing the xi-2 mutant seeds. We thank Dr. H. Tsukaya of the University of Tokyo and Dr. T. Nakano of RIKEN for suggestions about the phenotypic analysis. We also thank Dr. C. Saito, Dr. H. Abe, K. Shoda, E. Furuyama, K. Fukaya, R. Kiuch, E. Matsumoto, and M. Waga of the Nakano laboratory for assistance and suggestions. We thank the RIKEN BioResource Center (Tsukuba, Japan) for providing plasmids containing Arabidopsis calmodulin 3 (AT3G56800), which were developed by the plant genome project of the RIKEN Genomic Sciences Center ( Seki et al., 1998, 2002 ). This work was supported by Japan Society for the Promotion of Science KAKENHI grants 20001009 , 23770060 , and 24658002 ; the Futaba Electronics Memorial Foundation ; an Incentive Research Grant from RIKEN ; and the Japan Science and Technology Agency, PRESTO .
PY - 2013/11/11
Y1 - 2013/11/11
N2 - Cytoplasmic streaming is active transport widely occurring in plant cells ranging from algae to angiosperms. Although it has been revealed that cytoplasmic streaming is generated by organelle-associated myosin XI moving along actin bundles, the fundamental function in plants remains unclear. We generated high- and low-speed chimeric myosin XI by replacing the motor domains of Arabidopsis thaliana myosin XI-2 with those of Chara corallina myosin XI and Homo sapiens myosin Vb, respectively. Surprisingly, the plant sizes of the transgenic. Arabidopsis expressing high- and low-speed chimeric myosin XI-2 were larger and smaller, respectively, than that of the wild-type plant. This size change correlated with acceleration and deceleration, respectively, of cytoplasmic streaming. Our results strongly suggest that cytoplasmic streaming is a key determinant of plant size. Furthermore, because cytoplasmic streaming is a common system for intracellular transport in plants, our system could have applications in artificial size control in plants.
AB - Cytoplasmic streaming is active transport widely occurring in plant cells ranging from algae to angiosperms. Although it has been revealed that cytoplasmic streaming is generated by organelle-associated myosin XI moving along actin bundles, the fundamental function in plants remains unclear. We generated high- and low-speed chimeric myosin XI by replacing the motor domains of Arabidopsis thaliana myosin XI-2 with those of Chara corallina myosin XI and Homo sapiens myosin Vb, respectively. Surprisingly, the plant sizes of the transgenic. Arabidopsis expressing high- and low-speed chimeric myosin XI-2 were larger and smaller, respectively, than that of the wild-type plant. This size change correlated with acceleration and deceleration, respectively, of cytoplasmic streaming. Our results strongly suggest that cytoplasmic streaming is a key determinant of plant size. Furthermore, because cytoplasmic streaming is a common system for intracellular transport in plants, our system could have applications in artificial size control in plants.
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U2 - 10.1016/j.devcel.2013.10.005
DO - 10.1016/j.devcel.2013.10.005
M3 - Article
C2 - 24229646
AN - SCOPUS:84887539785
VL - 27
SP - 345
EP - 352
JO - Developmental Cell
JF - Developmental Cell
SN - 1534-5807
IS - 3
ER -