Cytosolic 20 alpha-hydroxysteroid dehydrogenase activity in spontaneous neoplasms in the dog and cat.

K. Shiota, N. Sasaki, N. Hattori, R. Miura, T. Hasegawa, J. X. Guang, K. Noda, M. Takahashi

Research output: Contribution to journalArticle

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Abstract

Steroid-producing tissues such as ovary, placenta, testis and adrenal gland and non-steroid-producing cells including lymphocytes are known to contain 20 alpha-hydroxysteroid dehydrogenase (20 alpha-HSD). In the present study, we measured and partially characterized the 20 alpha-HSD in 11 neoplastic tissues surgically removed from dogs and a cat. The tissues were pathologically classified as 4 benign and 7 malignant. The enzyme activities in the cytoplasmic fraction were positive in 6 of the 7 malignant tissues and 2 of the 4 benign ones. Following DEAE chromatography analysis of 2 malignant tumour samples, multiple forms of enzyme activities with different electric charges were detected. Furthermore, 20 alpha-HSD activity in the cytosol fraction from malignant tumours was increased dramatically by passage through the DEAE column, suggesting that the enzyme is present in the cytosol as an inactive or sequestered form.

Original languageEnglish
Pages (from-to)549-552
Number of pages4
JournalThe Journal of veterinary medical science / the Japanese Society of Veterinary Science
Volume53
Issue number4
Publication statusPublished - 1991 Aug
Externally publishedYes

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20-alpha-Hydroxysteroid Dehydrogenase
hydroxysteroid dehydrogenases
Cats
Dogs
cats
neoplasms
dogs
cytosol
Cytosol
Neoplasms
Enzymes
enzyme activity
electrical charges
adrenal glands
Adrenal Glands
placenta
Placenta
steroids
Testis
Chromatography

ASJC Scopus subject areas

  • Marketing
  • veterinary(all)

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Cytosolic 20 alpha-hydroxysteroid dehydrogenase activity in spontaneous neoplasms in the dog and cat. / Shiota, K.; Sasaki, N.; Hattori, N.; Miura, R.; Hasegawa, T.; Guang, J. X.; Noda, K.; Takahashi, M.

In: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 53, No. 4, 08.1991, p. 549-552.

Research output: Contribution to journalArticle

Shiota, K, Sasaki, N, Hattori, N, Miura, R, Hasegawa, T, Guang, JX, Noda, K & Takahashi, M 1991, 'Cytosolic 20 alpha-hydroxysteroid dehydrogenase activity in spontaneous neoplasms in the dog and cat.', The Journal of veterinary medical science / the Japanese Society of Veterinary Science, vol. 53, no. 4, pp. 549-552.
Shiota, K. ; Sasaki, N. ; Hattori, N. ; Miura, R. ; Hasegawa, T. ; Guang, J. X. ; Noda, K. ; Takahashi, M. / Cytosolic 20 alpha-hydroxysteroid dehydrogenase activity in spontaneous neoplasms in the dog and cat. In: The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 1991 ; Vol. 53, No. 4. pp. 549-552.
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