Cytotoxicity of synthetic barium hydroxyapatite

Masanori Kikuchi; Astsushi Yamazaki; Masaru Akao; Hideki Aoki

Cytotoxicity of synthetic barium hydroxyapatite

Masanori Kikuchi^*, Astsushi Yamazaki, Masaru Akao, Hideki Aoki

^*Corresponding author for this work

School of Creative Science and Engineering

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Barium hydroxyapatite (Ba₁₀(PO₄)₆(OH)₂, Ba-HAp) was synthesized by a wet method using Ba(OH)₂ · 8H₂O and (NH₄)₂HPO₄ as starting materials. The Ba-HAp obtained had a Ba/P atomic ratio of 1.76 and contained CO₃ groups. The Ba-HAp was sintered at 1073 K for 12 hours. The sintered Ba-HAp had a three point bending strength of 29 MPa and Young's modulus of 27 GPa. Cytotoxicity of the sintered bodies and particles was tested using L-cells. The sintered Ba-HAp showed no cytotoxicity, and the cells were closely in contact with the surfaces of sintered Ba-HAp. Morphological observation of the cell around the Ba-HAp particles also showed no cytotoxicity. However, cell growth was inhibited by Ca adsorption on the Ba-HAp particles. These results suggested that the Ba-HAp had no cytotoxicity and can be applied as a bioactive X-ray opaque material.

Original language	English
Pages (from-to)	405-413
Number of pages	9
Journal	Bio-Medical Materials and Engineering
Volume	6
Issue number	6
Publication status	Published - 1996 Dec 1

Keywords

Barium hydroxyapatite
Ca adsorption
Cell growth inhibition
Cytotoxicity
Mechanical strength

ASJC Scopus subject areas

Biomaterials
Biomedical Engineering

Cite this

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title = "Cytotoxicity of synthetic barium hydroxyapatite",

abstract = "Barium hydroxyapatite (Ba10(PO4)6(OH)2, Ba-HAp) was synthesized by a wet method using Ba(OH)2 · 8H2O and (NH4)2HPO4 as starting materials. The Ba-HAp obtained had a Ba/P atomic ratio of 1.76 and contained CO3 groups. The Ba-HAp was sintered at 1073 K for 12 hours. The sintered Ba-HAp had a three point bending strength of 29 MPa and Young's modulus of 27 GPa. Cytotoxicity of the sintered bodies and particles was tested using L-cells. The sintered Ba-HAp showed no cytotoxicity, and the cells were closely in contact with the surfaces of sintered Ba-HAp. Morphological observation of the cell around the Ba-HAp particles also showed no cytotoxicity. However, cell growth was inhibited by Ca adsorption on the Ba-HAp particles. These results suggested that the Ba-HAp had no cytotoxicity and can be applied as a bioactive X-ray opaque material.",

keywords = "Barium hydroxyapatite, Ca adsorption, Cell growth inhibition, Cytotoxicity, Mechanical strength",

author = "Masanori Kikuchi and Astsushi Yamazaki and Masaru Akao and Hideki Aoki",

year = "1996",

month = dec,

day = "1",

language = "English",

volume = "6",

pages = "405--413",

journal = "Bio-Medical Materials and Engineering",

issn = "0959-2989",

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TY - JOUR

T1 - Cytotoxicity of synthetic barium hydroxyapatite

AU - Kikuchi, Masanori

AU - Yamazaki, Astsushi

AU - Akao, Masaru

AU - Aoki, Hideki

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Barium hydroxyapatite (Ba10(PO4)6(OH)2, Ba-HAp) was synthesized by a wet method using Ba(OH)2 · 8H2O and (NH4)2HPO4 as starting materials. The Ba-HAp obtained had a Ba/P atomic ratio of 1.76 and contained CO3 groups. The Ba-HAp was sintered at 1073 K for 12 hours. The sintered Ba-HAp had a three point bending strength of 29 MPa and Young's modulus of 27 GPa. Cytotoxicity of the sintered bodies and particles was tested using L-cells. The sintered Ba-HAp showed no cytotoxicity, and the cells were closely in contact with the surfaces of sintered Ba-HAp. Morphological observation of the cell around the Ba-HAp particles also showed no cytotoxicity. However, cell growth was inhibited by Ca adsorption on the Ba-HAp particles. These results suggested that the Ba-HAp had no cytotoxicity and can be applied as a bioactive X-ray opaque material.

AB - Barium hydroxyapatite (Ba10(PO4)6(OH)2, Ba-HAp) was synthesized by a wet method using Ba(OH)2 · 8H2O and (NH4)2HPO4 as starting materials. The Ba-HAp obtained had a Ba/P atomic ratio of 1.76 and contained CO3 groups. The Ba-HAp was sintered at 1073 K for 12 hours. The sintered Ba-HAp had a three point bending strength of 29 MPa and Young's modulus of 27 GPa. Cytotoxicity of the sintered bodies and particles was tested using L-cells. The sintered Ba-HAp showed no cytotoxicity, and the cells were closely in contact with the surfaces of sintered Ba-HAp. Morphological observation of the cell around the Ba-HAp particles also showed no cytotoxicity. However, cell growth was inhibited by Ca adsorption on the Ba-HAp particles. These results suggested that the Ba-HAp had no cytotoxicity and can be applied as a bioactive X-ray opaque material.

KW - Barium hydroxyapatite

KW - Ca adsorption

KW - Cell growth inhibition

KW - Cytotoxicity

KW - Mechanical strength

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M3 - Article

C2 - 9138651

AN - SCOPUS:0030342678

SN - 0959-2989

VL - 6

SP - 405

EP - 413

JO - Bio-Medical Materials and Engineering

JF - Bio-Medical Materials and Engineering

IS - 6

ER -

Cytotoxicity of synthetic barium hydroxyapatite

Abstract

Keywords

ASJC Scopus subject areas

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