TY - JOUR
T1 - D-amino acid dipeptide production utilizing D-alanine-D-alanine ligases with novel substrate specificity
AU - Sato, Masaru
AU - Kirimura, Kohtaro
AU - Kino, Kuniki
N1 - Funding Information:
This study was supported in part by the 21C-COE Program “Practical Nano-Chemistry” from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, and by a Waseda University Grant for Special Research Projects (2004A-141).
PY - 2005
Y1 - 2005
N2 - D-Alanine-D-alanine ligase (Ddl) is an important enzyme in the synthesis of bacterial peptidoglycan. The genes encoding Ddls from Escherichia coli K12 (EcDdlB), Oceanobacillus iheyensis JCM 11309 (OiDdl), Synechocystis sp. PCC 6803 (SsDdl) and Thermotoga maritima ATCC 43589 (TmDdl), the genomic DNA sequences of which have been determined, were cloned and the substrate specificities of these recombinant Ddls were investigated. Although OiDdl had a high substrate specificity for D-alanine; EcDdlB, SsDdl and TmDdl showed broad substrate specificities for D-serine, D-threonine, D-cysteine and glycine, in addition to D-alanine. Four D-amino acid dipeptides were produced using EcDdlB, and D-amino acid homo-dipeptides were successfully produced at high yields except for D-threonyl-D-threonine.
AB - D-Alanine-D-alanine ligase (Ddl) is an important enzyme in the synthesis of bacterial peptidoglycan. The genes encoding Ddls from Escherichia coli K12 (EcDdlB), Oceanobacillus iheyensis JCM 11309 (OiDdl), Synechocystis sp. PCC 6803 (SsDdl) and Thermotoga maritima ATCC 43589 (TmDdl), the genomic DNA sequences of which have been determined, were cloned and the substrate specificities of these recombinant Ddls were investigated. Although OiDdl had a high substrate specificity for D-alanine; EcDdlB, SsDdl and TmDdl showed broad substrate specificities for D-serine, D-threonine, D-cysteine and glycine, in addition to D-alanine. Four D-amino acid dipeptides were produced using EcDdlB, and D-amino acid homo-dipeptides were successfully produced at high yields except for D-threonyl-D-threonine.
KW - D-alanine-D-alanine ligase
KW - D-amino acid dipeptide
KW - Dipeptide production
KW - Vancomycin-resistant enterococci
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U2 - 10.1263/jbb.99.623
DO - 10.1263/jbb.99.623
M3 - Article
C2 - 16233841
AN - SCOPUS:27644499529
SN - 1389-1723
VL - 99
SP - 623
EP - 628
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 6
ER -