Daily expression of mRNAs for the mammalian clock genes Per2 and Clock in mouse suprachiasmatic nuclei and liver and human peripheral blood mononuclear cells

Miyako Takata; Naoto Burioka; Shigehiro Ohdo; Hiroshi Takane; Hideyuki Terazono; Masanori Miyata; Takanori Sako; Hisashi Suyama; Yasushi Fukuoka; Katsuyuki Tomita; Eiji Shimizu

doi:10.1254/jjp.90.263

Daily expression of mRNAs for the mammalian clock genes Per2 and Clock in mouse suprachiasmatic nuclei and liver and human peripheral blood mononuclear cells

Miyako Takata, Naoto Burioka^*, Shigehiro Ohdo, Hiroshi Takane, Hideyuki Terazono, Masanori Miyata, Takanori Sako, Hisashi Suyama, Yasushi Fukuoka, Katsuyuki Tomita, Eiji Shimizu

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

The mammalian circadian clock is located in the suprachiasmatic nucleus (SCN) of the hypothalamus and in most peripheral tissues. Clock genes drive the biological clock. However, circadian expression variations of the human clock genes are still unclear. In this study, we analyzed the daily variations of mPer2 and mClock mRNA expression in both the mouse SCN and liver to evaluate the central and peripheral alterations in the rodent clock genes. We also examined whether there are the daily variations of the clock genes hPer2 and hClock in human peripheral blood mononuclear cells (PBMCs). The daily variation of mClock and mPer2 mRNA expression in mouse SCN and liver were determined at ZT2, ZT6, ZT10, ZT14, ZT18 or ZT22. We isolated PBMCs from 9 healthy volunteers at 9:00 and 21:00 and examined the expression of hPer2 and hClock mRNA by RT-PCR analysis. The animals exhibited a robust daily rhythm in the RNA levels of mPer2 in the SCN and liver (P

Original language	English
Pages (from-to)	263-269
Number of pages	7
Journal	Japanese Journal of Pharmacology
Volume	90
Issue number	3
DOIs	https://doi.org/10.1254/jjp.90.263
Publication status	Published - 2002 Nov 1
Externally published	Yes

Keywords

Clock gene
Liver
Per2
Peripheral blood mononuclear cell
Suprachiasmatic nucleus

ASJC Scopus subject areas

Pharmacology
Molecular Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1254/jjp.90.263

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Takata, M., Burioka, N., Ohdo, S., Takane, H., Terazono, H., Miyata, M., Sako, T., Suyama, H., Fukuoka, Y., Tomita, K., & Shimizu, E. (2002). Daily expression of mRNAs for the mammalian clock genes Per2 and Clock in mouse suprachiasmatic nuclei and liver and human peripheral blood mononuclear cells. Japanese Journal of Pharmacology, 90(3), 263-269. https://doi.org/10.1254/jjp.90.263

Takata, M, Burioka, N, Ohdo, S, Takane, H, Terazono, H, Miyata, M, Sako, T, Suyama, H, Fukuoka, Y, Tomita, K & Shimizu, E 2002, 'Daily expression of mRNAs for the mammalian clock genes Per2 and Clock in mouse suprachiasmatic nuclei and liver and human peripheral blood mononuclear cells', Japanese Journal of Pharmacology, vol. 90, no. 3, pp. 263-269. https://doi.org/10.1254/jjp.90.263

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abstract = "The mammalian circadian clock is located in the suprachiasmatic nucleus (SCN) of the hypothalamus and in most peripheral tissues. Clock genes drive the biological clock. However, circadian expression variations of the human clock genes are still unclear. In this study, we analyzed the daily variations of mPer2 and mClock mRNA expression in both the mouse SCN and liver to evaluate the central and peripheral alterations in the rodent clock genes. We also examined whether there are the daily variations of the clock genes hPer2 and hClock in human peripheral blood mononuclear cells (PBMCs). The daily variation of mClock and mPer2 mRNA expression in mouse SCN and liver were determined at ZT2, ZT6, ZT10, ZT14, ZT18 or ZT22. We isolated PBMCs from 9 healthy volunteers at 9:00 and 21:00 and examined the expression of hPer2 and hClock mRNA by RT-PCR analysis. The animals exhibited a robust daily rhythm in the RNA levels of mPer2 in the SCN and liver (P",

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AU - Takata, Miyako

AU - Burioka, Naoto

AU - Ohdo, Shigehiro

AU - Takane, Hiroshi

AU - Terazono, Hideyuki

AU - Miyata, Masanori

AU - Sako, Takanori

AU - Suyama, Hisashi

AU - Fukuoka, Yasushi

AU - Tomita, Katsuyuki

AU - Shimizu, Eiji

PY - 2002/11/1

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N2 - The mammalian circadian clock is located in the suprachiasmatic nucleus (SCN) of the hypothalamus and in most peripheral tissues. Clock genes drive the biological clock. However, circadian expression variations of the human clock genes are still unclear. In this study, we analyzed the daily variations of mPer2 and mClock mRNA expression in both the mouse SCN and liver to evaluate the central and peripheral alterations in the rodent clock genes. We also examined whether there are the daily variations of the clock genes hPer2 and hClock in human peripheral blood mononuclear cells (PBMCs). The daily variation of mClock and mPer2 mRNA expression in mouse SCN and liver were determined at ZT2, ZT6, ZT10, ZT14, ZT18 or ZT22. We isolated PBMCs from 9 healthy volunteers at 9:00 and 21:00 and examined the expression of hPer2 and hClock mRNA by RT-PCR analysis. The animals exhibited a robust daily rhythm in the RNA levels of mPer2 in the SCN and liver (P

AB - The mammalian circadian clock is located in the suprachiasmatic nucleus (SCN) of the hypothalamus and in most peripheral tissues. Clock genes drive the biological clock. However, circadian expression variations of the human clock genes are still unclear. In this study, we analyzed the daily variations of mPer2 and mClock mRNA expression in both the mouse SCN and liver to evaluate the central and peripheral alterations in the rodent clock genes. We also examined whether there are the daily variations of the clock genes hPer2 and hClock in human peripheral blood mononuclear cells (PBMCs). The daily variation of mClock and mPer2 mRNA expression in mouse SCN and liver were determined at ZT2, ZT6, ZT10, ZT14, ZT18 or ZT22. We isolated PBMCs from 9 healthy volunteers at 9:00 and 21:00 and examined the expression of hPer2 and hClock mRNA by RT-PCR analysis. The animals exhibited a robust daily rhythm in the RNA levels of mPer2 in the SCN and liver (P

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KW - Liver

KW - Per2

KW - Peripheral blood mononuclear cell

KW - Suprachiasmatic nucleus

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Daily expression of mRNAs for the mammalian clock genes Per2 and Clock in mouse suprachiasmatic nuclei and liver and human peripheral blood mononuclear cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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